Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Jilin University(Medicine Edition)》 2008-05
Add to Favorite Get Latest Update

Protective effect of Herba Leonuri on genetic damage and enhancement on lymphocyte function in mice

XING Shen-yang1,QIAO Ping2,WEN De-zhong3,ZHU Yu-zhuo3(1.Department of Animal Genetics and Breeding;College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Medical Genetics,School of Basic Medical Sciences,Jilin University,Changchun 130021,China)  
Objective To investigate the anti-mutation of Chinese medicinal herb Herba Leonuri and its effect on T lymphocyte proliferation in spleen.Methods The micronucleus test of mouse bone marrow cell(MNT) :thirty mice were divided into six groups(n= 5),negative control(NS),cyclophosphamide group(CP 3.0 mg·kg-1),Herba Leonuri antimutagenesis groups(Herba Leonuri with dosages of 1.0,2.0,4.0,8.0 g·kg-1+CP30 mg·kg-1).The improved method was used to detect the micronuclei frequency.Lymphocyte transformation test:twenty-four mice were divided into four groups(n=6),saline control,CP control(30 mg·kg-1),Herba Leonuri(2.0 g·kg-1),Herba Leonuri +CP(2.0 g·kg-1 Herba Leonuri +CP 30 mg·kg-1).MTT assay was used to calculate the stimulation index(SI).Results The micronuclei frequencies in Herba Leonur 2.0,4.0,8.0 g·kg-1 groups were lower than that in CP group(P0.05);the SI in saline control,Herba Leonuri,Herba Leonuri +CP,CP groups were 1.89±0.19,2.17±0.14,1.73±0.13 and 1.45±0.09,respectively,the difference of SI was significant between Herba Leonuri and saline control groups(P0.05);the difference of SI was significant between Herba Leonuri +CP and CP groups(P0.05).Conclusion Herba Leonuri may protect genetic material from injury caused by CP,namely,showing effective antimutagenesis and yet Herba Leonuri could enhance lymphocyte function.
【Fund】: 吉林省科技厅科技发展计划项目资助课题(200705364)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved