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《Journal of Jilin University(Medicine Edition)》 2009-04
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Changes of mitochondrial structure,ATPase and Ca~(2+) concentration in spermatogenic cells of mouse testes induced by low dose radiation

WANG Zhi-cheng,LIU Shu-chun,LI Peng-wu,KANG Shun-ai,LIANG Shuo,ZHAO Gang,GONG Shou-liang(MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China)  
Objective To observe the ultrastructure,ATPase activity and Ca2+ concentration(i) of mitochondria in the sperematogenic cells of mouse testes 3-24 h after low dose radiation with 0.025-0.200 Gy X-rays,and illuminate the effects of mitochondrion structure and relative biological function on apoptosis.Methods The ultrastructure changes of mitochondria in the spermatogenic cells were observed with transmission electron microscope;the ATPase activity was measured with protein enzymic method;i was measured indirectly by flow cytometry with Fluo-3 probes.Results The mitochondria swelled and vacuolizated,and their cristae were broken in the spermatogonia and spermatocytes 12 h after irradiation,and their nuclei were karyopyknosis,the acrosomal vesicle structure was ambiguity,the membrane structure was unclear,and the mitochondria in spermatids were vacuolization.The activities of Na+-K+-ATPase in mouse testis tissue 12 h after irradiated with 0.025-0.200 Gy decreased compared with those with 0 Gy,the Na+-K+-ATPase activities of the cells irradiated with 0.05-0.200 Gy decreased significantly compared with those with 0 Gy(P 0.05),and Ca2+-ATPase of the cells irradiated with 0.025-0.200 Gy decreased significantly compared with those with 0 Gy(P 0.05).The change of [Ca2+]i in mouse testis spermatogenic cells had similar dose-response relationship,[Ca2+]i after irradiated with 0.075 Gy decreased compared with those with 0 Gy(P 0.05).After 0.075 Gy irradiation for 3-24 h,the activities of Na+-K+-ATPase in mouse testis tissues decreased obviously compared with those at 0 h(P 0.05),the activities of Ca2+-ATPase in mouse testis tissues increased slightly at 3 h,then decreased at 6-24 h compared with those at 0 h(P 0.05);i in mouse testis spermatogenic cells had similar time course-response relationship,i at 12 h decreased significantly compared with at 0 h(P 0.05).Conclusion Low dose radiation could induce the structure changes in the spermatogenic cell mitochondria of mouse testes;at the same time,there are the regularities of dose-effect and time course-effect in the changes of ATPase activitiy and i induced by low dose radiation.
【Fund】: 国家自然科学基金资助课题(30870747)
【CateGory Index】: Q27
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