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《Journal of Jilin University(Medicine Edition)》 2011-02
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Influence of polysaccharide from schisandra chinensis in apoptosis of thyroid carcinoma SW579 cells and survivin gene expression

XU Ke-yu,XIAO Jian-ying(Department of Biochemistry and Molecular Biology,Liaoning Medical College,Jinzhou 121000,China)  
Objective To discuss the inhibitory effect of the polysaccharide from schisandra chinensis on human thyroid carcinoma SW579 cells and its mechanism.Methods 48 adult male Wistar rats were divided into negative control group and treatment group.The rats in negative control group were given 0.9%NaCl.The ones in treatment group were given the polysaccharide from schisandra chinensis pured for different doses.The SW579 cells were divided into control group,negative group and treatment group.The cells in control group were cultivated in RPMI-1640 medium containing 15% calf serum.The cells in negative group were cultivated in serum from the rats in negative control group;the cells in treatment group were cultivated in medium containing the rat serum in treatment group including low,medium and high dose drugs(100,200 and 400 mg·kg-1).The apoptosis of SW579 cells was measured by acridine orange under fluorescence microscope and flow cytometry.The proliferation of SW579 cells was detected by MTT assay.The expression of survivin was determined by Western blotting method.Results The flow cytometry results showed that the apoptotic rates in control group and negative control group were 4.23% and 4.10%;the apoptotic rates in low,medium and high dose treatment groups were 8.63%,35.63%,and 38.32%,respectively;there were significant differences between treatment groups and control group(P0.01).The MTT results showed that the inhibitory rate of cell growth in control group was(0.12±0.06)%.The inhibitory rates in low,medium and high dose treatment groups were(27.51±1.62)%,(34.69±0.79)%,and(45.83±1.33)%,respectively;there were significant differences between treatment groups and negative control group(P0.01).The expressions of survivin in low,medium and high dose treatment groups were lower than those in control group and negative congrol group(P0.01).Conclusion Polysaccharide from schisandra chinensis can inhibit the survivin expression in thyroid carcinoma SW579 cells and induce apoptosis of SW579 cells and inhibit cell proliferation
【Fund】: 辽宁省教育厅科研项目资助课题(2009A469)
【CateGory Index】: R736.1
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