Antioxidant effects of icariin and icariside Ⅱ in vitro

BAO Yu1,YANG Jian-xiong2,SUN Run-guang1(1.Biomedical Engineering Laboratory,School of Physics and Information Technology,Shaanxi Normal University,Xi'an 710062,China;2.Biochemistry and Molecular Biology Laboratory,School of Life Science,Shaanxi Normal University,Xi'an 710062,China)  

Objective To investigate the antioxidant activities of icariin and icariside Ⅱ,and to clarify the possible antioxidant mechanism.Methods Using Vc and butylated hydroxytoluene(BHT)as positive controls,the clearance rates of icariin,icariside Ⅱ and BHT on 1,1-diphenyl-2-picryl-hydrazyl radical(DPPH·),superoxide anion(O2-·) and hydroxyl free radical(OH·) were measured;the reduction rates of samples were detected by the system of potassium ferricyanide;the capacities of samples were detected by NADH-NBT-PMS system;the inhibitory rates of lipid peroxidation were detected by thiobarbituric acid(TBA) method;the total antioxidant activities were determined by bleaching of β-carotene suboleic acid system.Results The samples showed concentration-dependent scavenging capacities of DPPH·.The clearance abilities of icariside Ⅱ were higher than those of icariin(P0.05).The clearance effects of icariin and icariside Ⅱ were concentraion-dependent;compared with the same concentration of BHT,the O2-· clearance rates of icariside Ⅱ and icariin were slightly lower,and the clearance rate of icariin was much lower than that of icariside Ⅱ.The OH· clearance rates of icariin and icariside Ⅱ in the concentration of 0.1-0.5 g·L-1 were(16.76±0.35)%-(40.56±1.46)% and(15.65±0.72)%-(28.51±0.91)%.When the concentration was 0.9 g·L-1,the inhibitory rates of lipid peroxidation of icariin,Vc and icariside Ⅱ were(58.79±1.56)%,(75.05±2.12)% and(37.82±1.43)%.The inhibitory rates no longer changed with the dose increasing.As the sample concentration increasing,the reduction capacities of icariin,Vc and icariside Ⅱ showed concentration-dependent tendence.In 30-120 min the total antioxidant activity of icariside Ⅱ was lower than those of icariin and BHT(P0.05 or P0.01).The antioxidant activities of icariin and icariside Ⅱ in vitro were weaker than those of Vc and BHT.Conclusion Icariin and icariside Ⅱ shows obvious antioxidant activities in scavenging of DPPH·,O2-· and OH· and inhibition of lipid peroxidation,the total antioxidant activity and the reduction activity.

【Key Words】： icariin icariside Ⅱ free radical lipid peroxidation
【Fund】： 国家自然科学基金资助课题(10874108)
【CateGory Index】： R285

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