Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Jilin University(Medicine Edition)》 2014-04
Add to Favorite Get Latest Update

Inhibitory effect of transgiutaminase 2 on apoptosis of osteosarcoma cell line MG-63 and its mechanism

CAI Wen-tao;XIA Hong;SHENG Ning-jiang;LIN Ming-xia;Graduate School,Southern Medical University;Department of Orthopedics,Hainan Provincial People's Hospital;Department of Orthopedics,General Hospital,Guangzhou Military Command of PLA;  
Objective To investigate the anti-apoptosis effect of transgiutaminase 2(TG2) in osteosarcoma cell line MG-63 and to explore the mechanism of inhibiting apoptosis of tumor cells.Methods The TG2-tgrgeted siRNA was designed,and the hypoxia culture model of MG-63 cells was established by a hypoxia incubator and the cells were divided into four groups:normal oxygen group,the cells were cultured under normal oxygen;hypoxia group,the cells were cultured in hypoxic incubators;control siRNA hypoxia group,the cells were cultured in hypoxic incubators after transfection of control siRNA;TG2 siRNA hypoxia group,the cells were cultured in hypoxic incubators after transfection of TG2 siRNA.The expressions of Bax and cytochrome C(Cyt C)and the apoptotic rates were observed at different time(6,12,24,48,and 72h)after hypoxia culture.Microtiter plate assay was performed to monitor the intracellular TG2 activity.RT-PCR was used to detect the mRNA expressions of TG2 and Bax.The expressions levels of TG2,Bax and Cyt C were observed by immunohistochemical staining and Western blotting method.The apoptotic rates were analyzed using flow cytometry.Results Compared with normal oxygen group,the activity of TG2,the mRNA and protein expression levels of TG2 in hypoxia group and control siRNA hypoxia group were increased remarkably with the prolongation of the hypoxia time(P0.01);the expression level of Bax protein was decreased significantly(P0.01),but the expression level of Bax mRNA had no significant change(P0.05);the expression levels of Cyt C protein in cytoplasm and mitochondria and the apoptotic rates had no markedly changes(P0.05).Compared with hypoxia group and control siRNA hypoxia group,the expression levels of mRNA and protein of TG2 in TG2 siRNA hypoxia group were decreased significantly at different time points(P0.01);the protein expression levels of Bax and Cyt C in cytoplasm and the apoptotic rates were increased markedly(P0.01);the expression level of Cyt C in mitochondria was decreased(P0.01).Conclusion TG2 can inhibit the apoptosis of tumor cells through down-regulating the Bax expression and preventing Cyt C releasing into the cytoplasm.
【Fund】: 海南省科技厅自然科学基金资助课题(809048)
【CateGory Index】: R738.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHU Xun-min;TANG Juan-juan;LI Yin-yan;LIU Jia-xi;WEI Xue-mei;WANG Fei;CHEN Xiao-xiang;ZHANG Xue-yan;LI Zi;Guangzhou Medical University;;Association of tissue transglutaminase with invasive capacity of human non-small cell lung cancer cells and its mechanisms[J];The Journal of Practical Medicine;2013-21
2 LV Nanqian,FEI qing,ZHANG Qiyun,LOU Guoxiang.Department of Orthopaedics,Liqun Hospital,Shanghai 200333,China;The influence of chemokine receptor CXCR4 gene on the proliferation and migration of human osteosarcoma cells[J];Chinese Orthopaedic Journal of Clinical and Basic Research;2012-05
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China;Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP[J];Journal of The Fourth Military Medical University;2005-21
2 LIU Bing.De- partment of Clinical Laboratory,the 324th Hospital of PLA,Chongqing 400020,China;Expression of Bcl-2 and Caspase-3 protein in osteosarcoma and its clinical significance[J];International Journal of Laboratory Medicine;2009-06
3 ZHANG Shi-quan1,XIAO De-ming1,ZHU Yu-hua1,GUAN Hong2,LI Wei1,TAN Ji-feng11Department of Orthopeadics;2Depatrment of Pathdogy the Second People's Hospital of Shenzhen,Shenzhen 518035,China.;Protein expression of p53 in osteosarcoma and the correlation with clinical features and prognosis[J];Journal of Modern Oncology;2011-01
4 JIANG Dong1,ZHENG Shi-ying1,CHEN Suo-cheng2.(1.Department of Cardiothoracic surgery,First Affiliated Hospital of Soochow University,Suzhou 215006,China;2.Department of Cardiothoracic Surgery,First People's Hospital Affiliated to Jiangsu University,Zhenjiang 212002,China);Research Progress of Whole Body Hyperthermia and Apoptosis of Tumor Cells[J];Medical Recapitulate;2008-01
5 LI Zheng. People Hospital of Liaoning Province ( Liaoning Shenyang,110015);Expression and clinical significance of P53.Bcl-2 and Bax protein in human osteosarcoma[J];China Journal of Orthopaedics and Traumatology;2002-04
6 GAO Da-xin,LU Gang,ZHANG Hai-yan,et al.Department of Osteology,The First Affiliated Hospital,China Medical University,Shenyang 110001,China;The relationship between expression of Survivin mRNA and apoptosis in human osteosarcoma[J];Chinese Journal of Experimental Surgery;2004-03
7 GAO Da-xin*,ZHANG Hai-yan,L Gang,et al.*Department of Orthopaedics,The First Hospital of Qinhuangdao,Qinhuangdao 066000,China;Survivin antisense oligonucleotide combined with DDP in the treatment of nude mice bearing human osteosarcoma xenograft[J];Chinese Journal of Experimental Surgery;2005-08
8 QIU Zhiqiang, LIAO Qi. No.2 Department of Orthopedics, the Second Affiliated Hospital of Nanchang University, Nanchang Jiangxi, 330006, P.R.China.;PROGRESS OF OSTEOSARCOMA THERAPY[J];Chinese Journal of Reparative and Reconstructive Surgery;2010-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved