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STUDY ON ETHANOL TWO STEPS FERMENTATION BY IMMOBILIZED INULINASE & YEASTCELL USING INULIN AS RAW MATERIAL

MA Jia-jin,L~ǖ Yue-gang (College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100037, China)  
Studied the conditions of ethanol two steps fermentation by immobilized inulinase and Saccharomyces cerivisiae cell using inulin as raw material, and the optimum results are obtained.The study shows: when temperature is 28 ℃,pH=5.2,the sugar concentration of the feed is 20%,the flow-rate of feed of the continuous bioreactor is 42 mL/min,the fermentation rate is fastest, and the bio-conversion rate of ethanol is highest. When the fermentation time reaches ~24 h,) the bio-conversion rate achieves 96.6%, and the ethanol concentration of the fermentation brooth achieves 10.96%.
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