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《Journal of Beijing Technology and Business University(Natural Science Edition)》 2010-05
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BREEDING AND IDENTIFICATION OF CONJUGATED LINOLEIC ACID PRODUCING LACTIC ACID BACTERIA

ZHANG Jing,CHEN Cun-she,ZHANG Ying,HU De,REN Ya-lin(College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China)  
The ability of conjugated linoleic acid(CLA) formation by 13 lactic acid bacteria strains was studied and 8 strains had the ability to produce CLA which was determined by ultraviolet spectrophotometry.The strain As1.2686 showed highest ability of producing CLA,the concentration of CLA in fermentation broth was 13.63mg/L.The strain B11693 could produce 11.55mg/L of CLA in the fermentation broth.The strain B11693 was identified to be Lactobacillus brevis by analyzing the configuration,cell characteristics,physiological and biochemical characteristics,combining with 16SrDNA gene sequence analysis.
【Fund】: 北京市自然科学基金资助项目;; 北京市教育委员会科技计划重点项目
【CateGory Index】: TS201.3
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