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《JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE)》 1999-01
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TRANSGENIC TOMATOES WITH TOBACCO MOSAIC VIRUS 54×10~3 PROTEIN GENE AND THEIR RESISTANCE TO TMV INFECTION

Cheng Yinghao; Wang JiWei; Wang YingdianPan Naisui; Chen Zhangliang( Department of Biology Beijing Normal University,100857,Beijing China;2) Animal and Plant Quaratine Administration,100026,Beijing,China;The National Laboratory for ProteinEngineering and  
The cDNA encoding 54 x 103 protein of tobacco mosaic virus(TMV)is introducedinto tomato by Agrobacterium-mediated method and transgenic plants are obtained.Nopalinesynthtase activity is demonstrated in the kanamycin-resistance lantlet.It is demonstrated thatTMV 54 x 103 protein gene has been integrated into the genomes of tomato plants by PCR andNorthern blot. The transgenic tomatoes show no typical mosaic symptom after TMV infection inthe Whole life while the control plants show typical mosaic synptoms in 2 weeks after TMVinfection.
【Fund】: 国家重点实验室项目
【CateGory Index】: Q785
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