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《Journal of Beijing Normal University(Natural Science)》 2001-01
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STUDY ON THE INTERACTION OF BOVINE SERUM ALBUMIN AND FLEROXACIN BY FLUORESCENCE METHOD

Nie Lihua Zhao Huichun Wang Xuebin Wang Xu (Department of Chemistry, Beijing Normal University, 100875, Beijing, China)  
A fluorescence method is used to study the fluorescence quenching of bovine serum albumin by its interaction with fleroxacin. The interaction association constants of bovine serum albumin and fleroxacin are determined from a double reciprocal Lineweaver Burk plot. According to the Fster dipole dipole energy transfer, the distance to be measured between the fleroxacin and tryptophane is 4.37 nm. From thermodynamical coordination it can be judged that the binding power between fleroxacin and bovine serum albumin is static electric power.
【Key Words】: fluorescence quenching bovine serum albumin fleroxacin energy transition
【Fund】: 国家自然科学基金资助项目 !(2 99710 0 5 )
【CateGory Index】: O657.3
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