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《Journal of Beijing Normal University(Natural Science)》 2005-01
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METHOD FOR RNA ISOLATION FROM RICE CARYOPSES

Chen Xing Wang Qi Huang Yingchun Wang Xinsheng Wang Yingdian (College of Life Sciences, Beijing Normal University, 100875, Beijing, China)  
Total RNA is isolated from rice caryopses displaying different development status by using the methods of TRIZOL Regent, Guanidine Thiocyanate and improved SDS. The quality of total RNA is analyzed through gel electrophoresis and UV spectrometer. The results indicate that the RNA isolated by the improved SDS method shows clear bands of 28S rRNA and 18S rRNA, and the value of D 260 /D 280 is 1.84 to 1.97. RNA isolated by TRIZOL Regent and Guanidine Thiocyanate methods degrades and disperses in some degrees. RNA isolated by the improved SDS method is used for RT-PCR and a clear 1.7 kb band can be observed in agarose gel. It is also used for Northern blot, and shows specific signal. These results demonstrate that the quality and purity of the RNA obtained through the improved SDS method can meet the demands of molecular biology experiment.
【Fund】: 北京师范大学青年基金资助项目
【CateGory Index】: S511
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