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《Journal of Yellow Cattle Science》 2003-01
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Analysis on Sequence Polymorphism of mtDNA D-loop Region in Nanyang Cattle

LEI Chu zhao 1, CHEN Hong 1,2 , ZHANG Zhi qing 1 , YANG Gong she 1, LEI Xue qin 3, SUN Wei bin 1, Li Rui biao 1(1. College of Animal Science and Technology, Northwest Sci Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100,China;2.Institute of Biotechnology, Xuzhou Normal University, Xuzhou, Jiangsu 221009, China; 3. College of Animal Science and Technology, Henan Science and Technology University, Luoyang, Henan 471003, China)  
The mitochondrial DNA D loop region of Nanyang cattle was analyzed in this study. The results showed that there were three types of nucleotide haplotype of mtDNA D loop region in experimented Nanyang cattle. The average nucleotide mutational rates of the D loop region in Nanyang cattle No.1, No.2 and No.3 were 0.44%, 4.84% and 0.44%,respectively, and their average nucleotide mutational rate of hypervariable region was 0.81%, 7.57% and 0.00%,respectively. Only transition was found in mtDNA D loop region in Nanyang cattle No.1, transition and insertion were found in cattle No.3, and four types of nucleotide variation including transition, transversion, insertion and deletion were found in cattle No.2, of which the transition was frequent. Those indicated that rich nucleotide variational polymorphism of mtDNA D loop region existed in Nanyang cattle. After analyzing the bovine mtDNA D loop nucleotide haplotypes, it was possible that there were two maternal origins in Nanyang cattle population.
【Fund】: 教育部留学归国人员启动基金 ;; 国家高技术研究发展计划 (86 3计划 )项目 (2 0 0 1AA2 4 30 5 2 );; 国家自然科学基金 (30 0 70 5 5 1)资助
【CateGory Index】: S823.2
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