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《Journal Yellow Cattle Science》 2005-06
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Analysis of mtDNA Cyt b Gene Sequence Polymorphism in Qinchuan Cattle

CAI Xin~1 ZHANG Bao~1 LEI Chu-zhao~1 HU Shen-rong~1 SU Li-hong~1 CHEN Hong~(1,2) 1. College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Agricultural Molecular Biology,Yangling,Shaanxi 712100,China; 2. Institute of Cellular and Molecular Biology,Xuzhou Normal University,Xuzhou,Jiangsu 221116,China  
The complete sequences of mitochondrial Cyt b gene from six Qinchuan cattle were ana- lyzed.Comparison of these sequences revealed 19 polymorphic sites which were all transitions.Four hap- lotypes were defined from 19 variations.With the complete sequence of mtDNA cyt b for European cattle as a control,lower frequency of nucleotide variation was observed in haplotype No.1,No.2 and No.3,re- spectively,but higher frequency was found in No.4. Phylogenetic tree of six Qinchuan cattle was con- structed using UPGMA in MEGA.The results indicated that Qinchuan cattle were probably originated from two progenitors:Bos taurus and Bos indicus.Furthermore,Bos taurus had greater influence to Qinchuan cattle than Bos indicus did.
【Fund】: 国家自然科学基金(30471238) 国家863项目(2003AA243051) 西北农林科技大学拔尖人才支持计划项目资助。
【CateGory Index】: S823
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