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《China Journal of Traditional Chinese Medicine and Pharmacy》 2006-07
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Soluble Proteins SDS-PAGE of C.tubulosa(Schenk) R.Wight

Chen Pei Guo Yuhai Wang Baomin Zhai Zhixi(College of Agronomy and Bio-Technology,China Agricultural University,Beijing 100094)  
Objective: To identify 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers,and explore the relationship between electrophoretic fingerprint and identification on soluble-protein,meanwhile providing data for establishing electrophoresis pattern library of Chinese crude drugs.Methods: The electrophoretic characteristics of soluble-proteins from 5 specimens of C.tubulosa(Schenk) R.Wight on different parasitifers were studied with SDS-PAGE.Results: It indicated that reproducible and high resolvable electrophoresis patterns of soluble proteins of C.tubulosa(Schenk) R.Wight on different parasitifers by SDS-PAGE.Their degrees of polymorphism were 55.56%.Conclusion: Each specimen had its own unique band pattern distinguish from the others.The electrophoresis pattern of SDS-PAGE of soluble proteins can be regarded as protein fingerprints for identification of C.tubulosa(Schenk) R.Wight on different parasitifers.
【Fund】: 重大基础研究前期研究专项(No.2004CCA01200)
【CateGory Index】: R284
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