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《China Journal of Traditional Chinese Medicine and Pharmacy》 2007-08
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Effect of Radix Morindae Officinalis polysaccharide on expression of Cbfα1 mRNA in osteoblast cultured in vitro

LI Nan1 WANG He-ming1 GUO Su-hua1 ZHENG Liang-pu2 WANG Li1(1Fujian college of TCM,Fuzhou 350108,China;2Academy of integrative medicine,Fujian college of TCM,Fuzhou 350108,China)  
Objective: To investigate the effects of Radix Morindae Officinalis polysaccharide on expression of Cbfα1mRNA in osteoblast,and compared to its aqueous extract.Methods: Osteoblasts were separated from the parietal bone of 24-hours old SD rat,then cultured with Radix Morindae Officinalis polysaccharide or aqueous extract of Radix Morindae Officinalis in vitro.72 hours later,osteoblast Cbfα1mRNA levels were measured by RT-PCR method.Results: Osteoblasts Cbfα1mRNA level in Radix Morindae Officinalis polysaccharide group or aqueous extract of Radix Morindae Officinalis group are higher than those in control group. The expression of Osteoblasts Cbfα1mRNA in Radix Morindae Officinalis polysaccharide group is obviously higher than that in aqueous extract group.Conclusion: Both Radix Morindae Officinalis polysaccharide and aqueous extract of Radix Morindae Officinalis can increase the expression of osteoblast Cbfα1mRNA and the effect of Radix Morindae Officinalis polysaccharide is better than extract of Radix Morindae Officinalis.Which proved that Radix Morindae Officinalis polysaccharide is one of the active components to increase the activity of osteoblasts.
【Fund】: 国家自然科学基金(No.30271630);; 福建省青年科技人才创新项目资助(No.2003J039)
【CateGory Index】: R285.5
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