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《China Journal of Traditional Chinese Medicine and Pharmacy》 2007-12
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Study on protective effect and mechanism of rhubarb on intestinal barrier of piglet with sepsis

ZHANG Ying-qian1,LIU Jian-hua2,LI Dian-gui1,SUN Bo3(1Hebei Medical University,Shijiazhuang 050017,China;2 Hebei Provincial Children's Hospital,Shijiazhuang 050031,China;3 Children's Hospital of Fudan University,Shanghai 200032,China)  
Objective: To investigate the protective effect and mechanism of rhubarb on intestinal mucosa epithelial cells apoptosis of piglet with sepsis.Methods: The changes of apoptosis of intestinal mucosa epithelial cells were tested by flow cytometry and TUNLE.Molecular biology methods were used to observe the mRNA expression of zonula occludens(ZO-1),Occludin,TNF-α/IL-10 and the content of MDA,activity of SOD.Results: Compared with model group,the number of apoptosis cells in treatment group decreased apparently(P0.01),the mRNA expression of TNF-α in treatment group reduced significantly(P0.01),while the mRNA expression of IL-10 increased(P0.01),the level of MDA in treatment group decreased and SOD activity increased obviously(P0.01),and the mRNA expression of ZO-1、Occludin in treatment group increased obviously(P0.01).Under the electron microscope: zonula occludens were integrity relatively.Conclusion: Rhubarb can decrease the apoptosis of intestinal epithelial cells and promote the expression of zonula occludens by anti-inflammation,anti-oxidative damage,then to protect the mucosal barrier of pig with sepsis.
【Fund】: 河北省科技厅科技攻关计划项目(No.062761180)
【CateGory Index】: R285.5
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