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《China Journal of Traditional Chinese Medicine and Pharmacy》 2007-12
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Study on protective effect and mechanism of rhubarb on intestinal barrier of piglet with sepsis

ZHANG Ying-qian1,LIU Jian-hua2,LI Dian-gui1,SUN Bo3(1Hebei Medical University,Shijiazhuang 050017,China;2 Hebei Provincial Children's Hospital,Shijiazhuang 050031,China;3 Children's Hospital of Fudan University,Shanghai 200032,China)  
Objective: To investigate the protective effect and mechanism of rhubarb on intestinal mucosa epithelial cells apoptosis of piglet with sepsis.Methods: The changes of apoptosis of intestinal mucosa epithelial cells were tested by flow cytometry and TUNLE.Molecular biology methods were used to observe the mRNA expression of zonula occludens(ZO-1),Occludin,TNF-α/IL-10 and the content of MDA,activity of SOD.Results: Compared with model group,the number of apoptosis cells in treatment group decreased apparently(P0.01),the mRNA expression of TNF-α in treatment group reduced significantly(P0.01),while the mRNA expression of IL-10 increased(P0.01),the level of MDA in treatment group decreased and SOD activity increased obviously(P0.01),and the mRNA expression of ZO-1、Occludin in treatment group increased obviously(P0.01).Under the electron microscope: zonula occludens were integrity relatively.Conclusion: Rhubarb can decrease the apoptosis of intestinal epithelial cells and promote the expression of zonula occludens by anti-inflammation,anti-oxidative damage,then to protect the mucosal barrier of pig with sepsis.
【Fund】: 河北省科技厅科技攻关计划项目(No.062761180)
【CateGory Index】: R285.5
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Li-li1,ZHANG Ning2(1Suzhou Hospital of TCM of Jiangsu Province,Suzhou 215003,China;2Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China);Discussion on effect and proper time of enteroclysis in treating chronic renal failure[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-08
2 ZHOU Feng, WAN Bing, GAO Wei, et al.(Department of ICU in Hospital Affiliated to Jiangsu University, Zhenjiang, 212001, China);Initial Study of Radix et Rhizoma RHei’s Regulating Function and Its Mechanism on Severe Cases[J];Shanxi Journal of Traditional Chinese Medicine;2008-08
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Dai Dingwei, et al(Shanghai Institute for Pediatric Research, Xinhua Hospital,Shanghai second Medical University,Shanghai);Culture of Human Fetal Intestinal Epithelial Cell in Vitro[J];ACTA UNIVERSITATIS MEDICINALIS SECONDAE SHANGHAI;1996-05
2 CHEN Dechang,JING Bingwen,YANG Xingyi,et al.Changzheng Hospital,Second Military Medical University,Shanghai 200003;The protective effect of rhubarb on gut in critical illness[J];CHINESE CRITICAL CARE MEDICINE;2000-02
3 Chen Dechang,Jing Bing wen,Yang Jiandong,et al∥;Experimental and clinical studies of rhubarb on gastrointestinal dynamics[J];CHINESE CRITICAL CARE MEDICINE;1997-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Shaogen,Chen Xiaying,Wang Liji,Wang Changrong,Wang Yongjie (The First Affiliated Hospital,Anhui Medical University,Hefei 230022);EFFECT OF CODONOPSIS PILOSULA ON GASTROINTESTINAL MOTILITY IN Ⅲ  BURN RATS[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;1999-06
2 ;改良脐敷合灌肠治疗肝硬化难治性腹水诱发氮质血症的疗效观察[J];Journal of Nurses Training;2005-11
3 Ding Yuqin,He Zhijun,Du Chunling,etc(The Third Area of General Surgery,The Affiliated Lianyungang Hospital of Bengbu Medical College,Lianyungang,222023);Study on the effect of oral Chinese medicine combined by dilute Iodophor enema during abdominal surgery[J];Journal of Nurses Training;2009-01
4 SUN Jun jun,DING Ya fang,ZHU Tian lun.Department of General Surgery,The first Affiliated Hospital of Henan Sciences and Technology University Luoyang, Henan 471003, China);THE TREATMENT OF ENDOTOXEMIA FOR PORTAL HYPERTENSIVE CLRRHOSIS[J];Journal of Hepatobiliary Surgery;2003-02
5 Hu Sen,Xia Bin,Li Junyou,et al.Burn Institute,First Affiliated Hospital of General Hospital of PLA,Beijing,100037;The effects of Tongfu pellets (通腑颗粒) on gut tolerance to early enteral nutrition following intestinal ischemia and reperfusion in dogs[J];Infection,Inflammation,Repair;2006-04
6 YANG Tong,LIN Xi-pin,HUAGN Xian-wen.Department of Pediatrics,the Fifth Affiliated Hospital of Guangxi Medical University(Liuzhou 545001),China;Clinical studies of therapeutic effects of rhubarb on neonatal gastrointestinal dysfunction[J];Guangxi Medical Journal;2004-09
7 YANG Chunling, JIANG Qiuling;Related Factors and Nursing Care to Diarrhea in Critical Patients[J];Journal of Nursing Science;2007-07
8 ;Relationship between vary doses of rhubarb and acute jaundice hepatitis[J];Hunan Guiding Journal of Traditional Chinese Medicine and Pharmacology;2002-11
9 MA LiDepartment of Surgery,Zhengzhou Second People’s Hospital,Zhengzhou 450000,China;Preventive Effects of Rhubarb on Gastrointestinal Failure of Postoperation Following Serious Multiple Injuries[J];Journal of Medical Forum;2007-22
10 Hu Jiasheng,Cui Jie,Hua Wanlu,et al.The Hospital of Ma Anshan Iron and Steel Company,Ma Anshan 24300;Clinical study of treatment success rate and its relevant factors in multiple organ dysfunction syndrome[J];JOURNAL OF EMERGENOY MEDICINE;2000-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 JI Xiang-fu~1,SHI Dao-huz~2(1.Departmint of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;2.Department of Pharmacy,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,China);Advancces in Studies of Baicalin on Cardiovascular and Cerebrovascular Pharmacology[J];Strait Pharmaceutical Journal;2006-05
2 LUO Cheng-qun, ZHOU Jian-da, CHEN Dao-jin,et al. (Department of Burns and Plastics Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China);Effect of Chinese traditional medicine mixture on inflammatory response in rats with severe burn[J];;2004-01
3 TANG Dao-lin, SHI Yong-zhong, JIANG Lei, et al(Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410008, Hunan, China);Effects of Heat Shock Response on the Expression and LPS-induced Release of HMGB1[J];Journal of Elinical Research;2005-03
4 XIAO Xiang-cheng, ZHOU Qiao-ling ( Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China );Clinical Research of Ai Xi Te Combined with Coloclysis of Chinese Crude Drug on Treating Chronic Renal Failure[J];Journal of Elinical Research;2005-04
5 YAO Yong-ming, YAO Sheng, CHEN Jin-song et al. Burns Institute, 304 Hospital of PLA, Beijing 100037, China;Effects of NF-kappa B inhibition by pyrrolidine dithiocarbamate on tissue pro-and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis[J];Medical Journal of Chinese People's Liberation Army;2004-01
6 XU Cai-lin, YAO Yong-ming, YU Yan et al. Burns Institute, 304 Hospital of PLA, Beijing 100037, China;Effects of NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) on mRNA expression of high mobility group box-1 protein in organs of rats with endotoxic shock[J];Medical Journal of Chinese People's Liberation Army;2004-01
7 CHEN Dechang,LI Hongjiang,QIAO Lin,et al.Chang Zhen Hospital,Second Military Medical University,Shanghai 200003,China;The effect of rhubarb on oxygen radicals leakage from the mitochondria of rat liver in sepsis[J];Journal of Emergenoy Medicine;2002-02
8 WU Rong-lan, FENG Shun, WANG Ji-deSchool of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046,China;Study on the Inhibition Effect of Baicalin and Baicalin Metallic Coordination[J];Science & Technology Review;2006-02
9 ;大黄素对脓毒症大鼠保护作用的实验研究[J];Zhejiang Clinical Medical Journal;2007-05
10 ZHAO Gang 1,JIANG Wei 2,XU Hui fang 2 (1.Department of Anesthesiology,the Affiliated Hospital of Nanjing Railway Medical College, Nanjing 210009,China; 2.Department of Anesthesiology,the Sixth People Hospital of Shanghai City,Shanghai 200233,China);Protective Effect of Rhubarb on Inflammation Response Induced by Intestinal Ischemia-Reperfusion in Rats[J];Journal of Nanjing Railway Medical College;2000-04
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Chen Dechang,Jing Bingwen,Yang Xingyi et al.Changzheng Hospital,Second Military MedicaI University,Shanghai 200003;THE CURATIVE EFFECT OF RHUBARB ON GASTROINTESTINAL FAILURE[J];MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY;1996-01
2 Chen De chang;Yang Xing-yi;Jing Bing-wen,et al;Effect of rhubarb on stress injury of gastrointestinal mucosa in critical illness and its mechanism[J];Chinese Critical Care Medicine;1996-07
3 /CHEN Dechang,et al;Protective effect of rhubarb on gut barrier :An experi-mental study[J];CHINESE CRITICAL CARE MEDICINE;1994-06
4 Chen Dechang,Yang Jiandong,Jing Bingwen,et al∥;Effect of rhubarb on permeability changes in intestinal mucosa and capillary[J];CHINESE CRITICAL CARE MEDICINE;1997-07
5 Chen Dechang,Jing Bing wen,Yang Jiandong,et al∥;Experimental and clinical studies of rhubarb on gastrointestinal dynamics[J];CHINESE CRITICAL CARE MEDICINE;1997-07
6 Yang Jiandong,Chen Dechang,Jing Bingwen,et al.Department of Emergency,Changzheng Hospital,Second Military Medical University,Shanghai 200003;The influence of rhubarb on phospholipase A 2 and platelet activation factor release in rats after endotoxic shock[J];CHINESE CRITICAL CARE MEDICINE;1998-08
7 Chang Zhen HosptialChen Dechang,et al.;The Effect of Rhubarb on Intestinal Permeability of Rats with Hemorrhagic Shock[J];;1994-03
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