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《China Journal of Traditional Chinese Medicine and Pharmacy》 2008-01
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Study on quick extraction and determination of icariin from Herba Epimedii

JIANG Ning1,LIU Xiao-peng2(1School of Biological Science and Technology,Hubei College of Nationalities,Enshi 445000,China;2Hubei Key Laboratory of Biological Resource Conservation and Utilization,Enshi 445000,China)  
Objective: To study the quick extraction and determination of icariin in Herba Epimedii.Methods: The orthogonal design and ASE300 system were used to study the extraction method of icariin.The content of icariin was determined by HPLC.The methanol water glacial acetic acid(60∶40∶0.25) was used as a mobile phase. The flow rate was 1.0ml/min,the detection wavelength was 270nm and the column temperature was 41℃.Results: The optimal extraction conditions were: 50% alcohol and extracting one times for twenty minutes each time at 70℃.The extracting rate of icariin was 93.15%.The RP-HPLC for determining method is simple,rapid and accurate.Conclusion: The extraction and determination methods are practicable for icariin in Herba Epimedii.
【Key Words】： Icariin Extraction RP-HPLC Content
【CateGory Index】： R284
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