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《China Journal of Traditional Chinese Medicine and Pharmacy》 2009-02
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Studies on hippocampus proteome of rats with syndrome of spleen yin deficiency by two-dimensional gel electrophoresis

ZHAN Li-bin1,LIU Li2,LU Xiao-guang2,GONG Xiao-yang3(1Department of TCM,the Second Affiliated Hospital of Dalian Medical University,Dalian 116023,China;2Emergency Department of Affiliated Zhongshan Hospital of Dalian University,Dalian 116001,China;3Department of TCM,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China)  
Objective:To apply two dimensional gel electrophoresis(2-DE) to initially analyze the protein expression in hippocampus of rats with spleen yin deficiency syndrome and to get the theory foundation for investigating the molecular mechanisms of Alzheimer's Disease and spleen yin deficiency syndrome.Methods:Rats model of spleen yin deficiency were set up by combination of improper diet,overstrain and consumption of body fluid.Qualitative changes of the proteome expression in hippocampus were compared between the model group and control group by two-dimensional gel electrophoresis.Results:The model group of spleen yin deficiency had the appearance of spleen yin deficiency,for example,irritability,increment in biting frequency,increase of rectal temperature and drinking water(P0.05) . There were obvious differences in expression of six protein spots between the model group and control group.Five protein spots showed lower expression and one spot increased significantly.Conclusions:The changes discovered by 2-DE in expression level and modification of some proteins may contribute to learning and memory abilities.
【Fund】: 国家自然科学基金资助项目(No.30472255)
【CateGory Index】: R256.3
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