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《China Journal of Traditional Chinese Medicine and Pharmacy》 2010-03
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Content determination of Podophyllotoxin in two preparations

JIANG Xiao-hong1,HUANG Huan1,HUANG Xiong1,JIN Chen-sheng2(1Medical College of Jiaxing University,Jiaxing 314001,China;2The Second Hospital of Jiaxing,Jiaxing 314000,China)  
Objective:To develop a HPLC method for determination of Podophyllotoxin in two preparations.Methods:HPLC was carried out,using a Dikma diamonsil C18 column(4.6mm×150mm,5μm)with a column temperature at 25℃,the methanol-water as mobile phase(using gradient)with flowing rate 1.0 mL/min.The detection wavelength was at 292 nm.Results:The method had good linear relationship within the range of 11.8-590μg/mL for Podophyllotoxin,with the correlation coefficients 0.9999.The results of the average recovery of Youdi liniment and Podophyllotoxin tincture were 99.38%,99.62%.Conclusion:The rapid,accurate and reproducible RP-HPLC method was useful for quantification of Podophyllotoxin in Youdi Liniment and Podophyllotoxin Tincture.
【Fund】: 浙江省自然科学基金(No.Y2081030)~~
【CateGory Index】: R286.0
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