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《China Journal of Traditional Chinese Medicine and Pharmacy》 2011-02
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Electric acupuncture intervention in angiogenesis and damaged cells in hypertensive rats with cerebral ischemia and reperfusion

WAN Sai-ying1,TAN Feng1,WU Hai-ke1,WANG Jin-liang1,LI Yan-ping1,CHENG Jia-mao2 (1Departmengt of Neurology,Foshan Hospital of TCM,Guangzhou University of Chinese Medicine,Foshan 528000,China;2Laboratory of Neurology,Zhongshan University,Guangzhou 521000,China)  
Objective: To investigate the influence of electric acupuncture on the expression of angiopoietin-1(Ang-1),thrombospodin-1(TSP-1) and angiogenesis during the different time of the cerebral ischemia and reperfusion in Stroke-prone-Renovascular-Hyper-tensive-Rats(RHRSP).Methods: 120 Renovascular hypertensive rats with stroke-prone(RHRSP) models were established by bilaterally narrowing the renal artery with sliver loop clips and then randomly divided into four groups: blank group(12),sham-operated group(12),model group(48) and electric acupuncture group(48).The middle cerebral artery occlusion(MCAO) models were duplicated by thread occlusion method in model group and electric acupuncture group.EA at Bai Hui and Da Zhui points were carried out.in electric acupuncture group once a day for 14 days.The behavioral scores and ultrastructural changes of rats were observed at each time point.The expression of Ang-1,TSP-1 and changes of microvessel density(MVD) in ischemic area of rats were detected by immunohistochemistry,electron microscope on 1th,7th,14th,28th days 2h after cerebral ischemia and reperfusion.Results: The behavioral scores of rats in electric acupuncture group decreased significantly than that in model group(P0.05) on 1th,7th,28th days.The brain water content of rats in electric acupuncture group and model group increased obviously than that in blank group and sham-operated group,and it was higher in contral group than that in electric acupuncture group on 1th,7th days.Compared with model group,the expression of Ang-1 was strengthened(P0.05) on 7th,14th,28th days but TSP-1 was lowered(P0.05) on 1th,7th,14th days in electric acupuncture group.The microvessel density in and around ischemic area increased obviously in electric acupuncture group than those in contral group(P0.05) on 14th,28th days.The injury of neurons and endothelial cells in electric acupuncture group was more obviously alleviated than that in model group at each time point.Conclusion: The mechanism of electric acupuncture protects RHRSP against the injury of the cerebral ischemia and reperfusion may be related to up-regulating the expression of Ang-1,down-regulating TSP-1 and promoting the angiogenesis in ischemic area.
【Fund】: 广东省中医药管理局基金(No.1050006);; 广东省自然科学基金(No.8152800007000001)~~
【CateGory Index】: R245
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 XUE Yu-xian1,GAO Jian-feng2,3,LIU Ke3(1Department of Pathology of Henan Medical College for Staffs and Workers,Zhengzhou 450002,China;2The Third Hospital Affiliated to Zhengzhou University,Zhengzhou 450052,China;3Henan College of TCM,Zhengzhou 450008,China);Effect of electro-acupuncture on amino acid transmitter and NF-κB expression in aged rats with focal cerebral ischemia reperfusion injury[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Tong1,ZHANG Li1,ZHANG Hui-min2,LI Qian1(1School of Acupuncture & Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;2School of Preclinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China);Systematic review of acupuncture therapy for acute ischemic stroke[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-01
2 FAN Ling,CHANG Xiao-rong,YAN Jie,ZENG Jun,WANG Chao(TCM University of Hunan,Changsha,Hunan 410007,China);Effect of electroacupuncture on NO,NOS and ET-1 in serum of rats with acute cerebral ischemia[J];Journal of Traditional Chinese Medicine University of Hunan;2008-01
3 WANG Jun,MA Kai,TU Wai-qian (Henan Academy of Traditional Chinese Medicine,Zhengzhou 450004,Henan,China);Effect of Electroacupuncture on Excitatory Amino Acid in Brain Tissue of Rat with Cerebral Ischemia Reperfusion[J];Liaoning Journal of Traditional Chinese Medicine;2007-02
4 DU Yixu XU Nenggui YI Wei HUANG Guoxin CAI Tong HUANG Zhenyan WU Lingni (Guangzhou University of TCM,Guangzhou 510405 Guangdong,China);Effect of Electroacupuncture on the Synaptic Number Density and Surface Density in the Cerebral Ischemic Areas of Rats with Focal Cerebral Ischemia at Different Time[J];Journal of Guangzhou University of Traditional Chinese Medicine;2008-03
5 HUANG Xiao-jie,YAN Yao-dong,XIE Ning,QIN Hai-jun,XIONG Lü,WANG Ling-ling(Affiliated Hospital of Institute of Aviation Medicine,Air Force,Beijing,100089);Observation on Therapeutic Effect of Acupuncture Combined with Chinese Herbal Medicine in Sequela of Wind Stroke[J];World Journal of Integrated Traditional and Western Medicine;2008-12
6 LIU Hua, WANG Yi, CHANG Lu hua (Dept. of Neurology, the First Affiliated Hospital of Kunming Medical College, Kunming, 650031);Development of Neurobiochemical Researches on Acupuncture Treatment of Ischemic Stroke[J];Acupuncture Research;2004-01
7 CHEN Xiao-yu,ZHANG Qiu-ling,BAI Bo(Department of Neurobiology,Taishan Medical College,Tai′an 271000,China);Effect of Electroacupuncture on Mitochondrial Membrane Potential and Apoptosis in the Cerebral Cortex in Rats with Focal Cerebral Ischemia/Reperfusion Injury[J];Acupuncture Research;2008-02
8 HAN Miao-miao MIN Lian-qiu SONG Xiao-wei (Department of Neurology,the First Affiliated Hospital of Liaoning Medical College,Jinzhou LIAONING 121001,China);Effect of procyanidin on excitatory amino acids of hippocampus in type 2 diabetes mellitus rats with focal cerebral ischemia[J];Chinese Journal of New Drugs and Clinical Remedies;2008-10
9 WU Jun-Fang;SHI Yi-Ju; LIU Tian-Pei(Department of Pharmacology,Nanjing Medical University,Nanjing 210029;Department of Pharmacology,Tai'an Medical College,Tai'an 271000);Protective effcts of berberine on cerebral ischemia in mice and rats[J];CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY;1995-02
10 LI Peng (The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China);Problems in studies of acupuncture for treatment of ischemic cerebrovascular diseases in recent years[J];Chinese Acupuncture & Moxibustion;2007-02
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