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《China Journal of Traditional Chinese Medicine and Pharmacy》 2011-02
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Electric acupuncture intervention in angiogenesis and damaged cells in hypertensive rats with cerebral ischemia and reperfusion

WAN Sai-ying1,TAN Feng1,WU Hai-ke1,WANG Jin-liang1,LI Yan-ping1,CHENG Jia-mao2 (1Departmengt of Neurology,Foshan Hospital of TCM,Guangzhou University of Chinese Medicine,Foshan 528000,China;2Laboratory of Neurology,Zhongshan University,Guangzhou 521000,China)  
Objective: To investigate the influence of electric acupuncture on the expression of angiopoietin-1(Ang-1),thrombospodin-1(TSP-1) and angiogenesis during the different time of the cerebral ischemia and reperfusion in Stroke-prone-Renovascular-Hyper-tensive-Rats(RHRSP).Methods: 120 Renovascular hypertensive rats with stroke-prone(RHRSP) models were established by bilaterally narrowing the renal artery with sliver loop clips and then randomly divided into four groups: blank group(12),sham-operated group(12),model group(48) and electric acupuncture group(48).The middle cerebral artery occlusion(MCAO) models were duplicated by thread occlusion method in model group and electric acupuncture group.EA at Bai Hui and Da Zhui points were carried out.in electric acupuncture group once a day for 14 days.The behavioral scores and ultrastructural changes of rats were observed at each time point.The expression of Ang-1,TSP-1 and changes of microvessel density(MVD) in ischemic area of rats were detected by immunohistochemistry,electron microscope on 1th,7th,14th,28th days 2h after cerebral ischemia and reperfusion.Results: The behavioral scores of rats in electric acupuncture group decreased significantly than that in model group(P0.05) on 1th,7th,28th days.The brain water content of rats in electric acupuncture group and model group increased obviously than that in blank group and sham-operated group,and it was higher in contral group than that in electric acupuncture group on 1th,7th days.Compared with model group,the expression of Ang-1 was strengthened(P0.05) on 7th,14th,28th days but TSP-1 was lowered(P0.05) on 1th,7th,14th days in electric acupuncture group.The microvessel density in and around ischemic area increased obviously in electric acupuncture group than those in contral group(P0.05) on 14th,28th days.The injury of neurons and endothelial cells in electric acupuncture group was more obviously alleviated than that in model group at each time point.Conclusion: The mechanism of electric acupuncture protects RHRSP against the injury of the cerebral ischemia and reperfusion may be related to up-regulating the expression of Ang-1,down-regulating TSP-1 and promoting the angiogenesis in ischemic area.
【Fund】: 广东省中医药管理局基金(No.1050006);; 广东省自然科学基金(No.8152800007000001)~~
【CateGory Index】: R245
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