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Protective effect of baicalin on H_2O_2 injured rats' primary cortical neurons

CHENG Fa-feng1,SONG Wen-ting2,GUO Shao-ying1,ZHONG Xiang-gen1,LU Yi1,WANG Qing-guo1(1School of Preclinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;2Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)  
Objective:To explore the protective effect of baicalin on H2O2 injured rats' primary cortical neurons.Methods:H2O2 was employed to form the injured rats' primary cortical neuron model,and the antioxidative effect of Baicalin was examined by this model.The free radical scavenging effects of different concentrations of baicalin were measured with DPPH method.The superoxide anion resistance detected by kits.The effect of baicalin on xanthine oxidase activity was measured by detecting the content of uric acid produced in xanthine/xanthine oxidase system.Results:Baicalin improved the livability of H2O2 injured rats' primary cortical neurons,superoxide dismutase activity was increased and lactate dehydrogenase was reduced in the neuron culture.Baicalin could scavenge free radical and superoxide anion;in addition,it could inhibit the activity of xanthine oxidase,and the content of uric acid,produced in xanthine/xanthine oxidase system,continuously reduced following increasing baicalin concentrations.Conclusion:Baicalin can exert protective effect on H2O2 injured rats' primary cortical neurons,and this effect could be related to increasing SOD activity,scavenging free radical and superoxide anion directly,and inhibiting xanthine oxidase activity.
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