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《China Journal of Traditional Chinese Medicine and Pharmacy》 2011-08
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Effect of pseudoephedrine and ephedrine on the activities of cytochrome P450 enzymes in rat liver microsomes

WU Wen-hua,LIU Li,HAN Feng-mei,CHEN Yong(Hubei Province Key Laboratory of Biotechnology of Chinese Medicine,Hubei University,Wuhan 430062,China)  
Objective:To study the effect of ephedrine and pseudoephedrine on the activities of cytochrome P450 enzymes(CYP450s) in rat liver microsomes.Method:The probe substrate of CYP450 enzymes:diclofenac for CYP2C6,omeprazole for CYP2C,phenacetin for CYPlA2,chlorzoxazone for CYP2E1,testosterone for CYP3A,was individually co-incubated with different concentration of ephedrine and pseudoephedrine in rat liver microsomes.Then the relative metabolic clearance rate(RMCR) of each probe substrate was determined by HPLC method.Results:The IC50 values of pseudoephedrine for the activities of CYP1A1/2 and CYP2E1 were 54.0 and 206.7μmol/L respectively.Whereas at concentration up to 400μmol/L,ephedrine promoted the RMCR of phenacetin and diclofenac up to 2.3-fold and 1.6-fold respectively.Conclusion:Pseudoephedrine has inhibitory action on the activities of CYP1A1/2 and CYP2E1,whereas ephedrine has induction on the activities o f CYP2C and CYP1A1/2 in some extent.
【Fund】: 湖北省高等学校优秀中青年团队计划项目(No.T200807)~~
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 GUO Yu, WANG Hui (Dept of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071,China);Study of probe substrate specificity for human cytochrome P450 isoenzymes[J];Chinese Pharmacological Bulletin;2007-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GAO Ling ZHANG Xue-ming YAO Qing CAO Xiu-qin(Ningxia Medical College,Yinchuan 750004,China);Study on antipyretic action of dipseudoephedrine glycyrrhizin and its effect on heart rate and blood pressure of rats[J];China Journal of Traditional Chinese Medicine and Pharmacy;2007-11
2 WU Li-yan1, CHEN Zhi-yun2, YAN Mao-xiang2, HE Bei-hui2(1Zhejiang University of TCM, Hangzhou 310006, China; 2Zhejiang TCM Hospital, Hangzhou 310006, China);Effect of Sanchi on expression of hepatic CYP2E1 in liver tissues in rats with alcoholic liver disease[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-06
3 XIN Wei,ZOU Mei-juan,ZHENG Dan,LI Su,CHENG Gang(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China);Preparation and dissolution research of compound pseudoephedrine hydrochloride sustained-release pellets[J];Journal of Shenyang Pharmaceutical University;2007-10
4 PANG Feng-juan1,ZOU Mei-juan1,LIU Gou-liang2,XUE Xiao-bai1,LU Xin-xin1,CHENG Gang1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Harbin Taihua Pharmaceutical Co.,Ltd.,Harbin 150302,China);Optimization of coating solution formulation of pseudoephedrine hydrochloride pulsatile-release mini-tablets[J];Journal of Shenyang Pharmaceutical University;2009-03
5 SUN Peng,JIN Ying-shun (Department of Pharmacology,Affiliated Hospital of Yanbian University,Yanji 133000,China);Rapid Determination of Cytochrome P450 1A2 Activity with Phenacetin Probe Using High-performance Liquid Chromatography[J];Lishizhen Medicine and Materia Medica Research;2008-12
6 BI Hui-chang 1, CHEN Xiao 2 , HUANG Min 1 (1.Institute of Clinical Pharmacology, School of Pharmaceutical Sciences;2.Dept of Pharmacy, The First Affiliated Hospital , Sun Yat-sen University, Guangzhou 510080, China);Liver perfusion technique and its applications in drug study[J];Chinese Pharmacological Bulletin;2004-09
7 CHEN Hao,TAN Zhi-rong,ZHOU Hong-hao(Institute of Clinical Pharmacology,Central South University,Changsha 410078,China);Advance in flavin-containing monooxygenase 3(FMO3):structure/function and genetic polymorphisms[J];Chinese Pharmacological Bulletin;2008-10
8 AI Chang-hong1,2,SUN Han-xiong1,LI Hua1,ZHUANG Xiao-mei1,DONG De-li2(1.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China; 2.Dept of Pharmacology,Haerbin Medical University,Haerbin 150081,China);In vitro inhibition of cytochrome P450 activities by active constituents of Chinese herbal drugs[J];Chinese Pharmacological Bulletin;2011-04
9 LV Xia1,GUO Qing2,ZHONG Wen-ying1 1Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009; 2Jiangsu Institute for Food & Drug Control,Nanjing 210008,China;GC-MS determination of pseudoephedrine,ephedrine,strychnine and brucine in Shufeng Huoluo pills[J];Journal of China Pharmaceutical University;2008-06
10 ZHANG Ning1,YANG Xian-le1,2,WANG Xiang-ling1,LIN Mao3,YU Wen-juan1,ZHANG Shu-jun1(1.Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China;2.E-Institute of Shanghai Municipal Education Commission,Shanghai 200090,China;3.Fisheries College,Jimei University,Xiamen 361021,China);Induced effect of ethanol on cytochrome P450 2E1 in CHSE-1 cell line[J];Journal of Fishery Sciences of China;2007-05
China Proceedings of conference Full-text Database 2 Hits
1 YANG Zhi-fu①, TIAN Yun①, YANG Jing①, CHEN Min-chun①, QIAO Yi②, JIA Yan-yan①, GUAN Yue①, WEN Ai-dong①* (①Department of Pharmacy, Xingjing Hospital,Fourth Military Medical University Xi’an 710032, ②College of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712046);Simultaneous evaluation of acitivity of hepatic CYP450 in acute blood stasis rats by cocktail probe drugs[A];[C];2009
2 YANG Zhi-fu①,TIAN Yun①,YANG Jing①,CHEN Min-chun①,QIAO Yi②,JIA Yan-yan①,GUAN Yue①,WEN Ai-dong① (①Department of Pharmacy,Xingjing Hospital,Fourth Military Medical University Xi’an 710032,②College of Pharmacy,Shaanxi University of Chinese Medicine Xianyang 712046);Simultaneous evaluation of acitivity of hepatic CYP450 in acute blood stasis rats by cocktail probe drugs[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 PENG Wen-Xing, WANG Xin-Liang, LI Huan-De (Clinical Pharmacy Research Laboratory,The Xiangya 2nd Hospital, Centralsouth University, Changsha 410011) (Basical and Clinical Pharma- cology Research Institute Xiangya Medical School, Centralsouth University,;Drug-drug interactions mediated by p-glycoprotein[J];The Chinese Journal of Clinical Pharmacology;2001-05
2 YANG TSAO-CHEN JANG CH'ANG-SHAW (Department of Pharmacology, First Medical College of Shanghai, Shanghai);STUDIES ON THE MECHANISM OF TACHYPHYLAXIS TO EPHEDRINE[J];Acta Physiologica Sinica;1963-04
3 SHU HUAI-DE(Department of Pharmacology,Shanghai First Medical College);ACTIONS OF EPHEDRINE ON SMOOTH MUSCLE OF MOUSE VAS DEFERENS[J];Acta Physiological Sinica;1983-02
4 GONG QIN-YAN;YANG ZAO-CHEN(Department of Pharmacology,Shanghai First Medical College);STUDIES ON THE MECHANISM OF EPHEDRINE ON RABBIT AORTA AND ATRIUM[J];Acta Physiological Sinica;1984-04
5 SHU HUAI-DE;JIN BEI;YANG ZAO-CHEN Department of Pharmacology, Faculty of Basic Medical Science, Shanghai Medical University;THE MECHANISM OF INHIBITORY ACTION OF EPHEDRINE ON THE TWITCH RESPONSE OF MOUSE VAS DEFERENS TO FIELD STIMULATION[J];Acta Physiological Sinica;1986-06
6 BI Hui-chang 1, CHEN Xiao 2 , HUANG Min 1 (1.Institute of Clinical Pharmacology, School of Pharmaceutical Sciences;2.Dept of Pharmacy, The First Affiliated Hospital , Sun Yat-sen University, Guangzhou 510080, China);Liver perfusion technique and its applications in drug study[J];Chinese Pharmacological Bulletin;2004-09
7 LIU Yan~1,HUANG Li-jun~1,WANG Zhen~1,ZHANG Yi-fei~1,ZHU Da-ling~(1,2)(1.Institute of Clinical Pharmacy,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China;2.College of Pharmacy of Harbin Medical University,Bio-pharmaceutical Key lab of Heilongjiang Province,Harbin 150086,China);Effect of nizatidine on rat CYP1A2 expression and activity[J];Chinese Pharmacological Bulletin;2006-07
8 ZHU Ming-yian, ZHAO Nai-cai (Dept Pharmacology, China Medical University, Shen Yang 110001);Mechanism of ephedrine action on pulmonary artery strips of rabbits[J];Chinese Journal of Pharmacology and Toxicology;1987-03
9 CHEN Jie, PENG Ren-Xiu~*, YANG Jing, LU Jing-Feng (Department of Pharmacology, Medical College, Wuhan University, Wuhan 430071, China);Mechanism of combined action of 18 α-diammonii glycyrrhizinatis and glibenclamide on glucose metabolism in experimental diabetic rats[J];Chinese Journal of Pharmacology and Toxicology;2005-04
10 GUO Yu, WANG Hui Department of Pharmacology, Medical College of Wuhan University, Wuhan 430071, Hubei, China;Establishment of precision-cut fibrotic liver slice technique[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2004-08
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