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Effect of preventive moxibustion at Mingmen(DU-4) on swimming exhausted time and HPA axis hormone of rats

SUN Zhi-fang1,LI Xiao-hong1,MO Jie1,WANG Jing1,HE Long1,DING Na1,WANG Hong-bin2,ZHANG Lu-fen1(1Acunpunture school of Beijing University of Chinese Medicine,Beijing 100029,China;2Teaching and Research Division of Acupuncture and Moxibustion and Tuina of TCM School of Hebei United University,Tangshan 063000,China)  
Objective: To observe the effect of preventive acupuncture at Mingmen(DU-4) on exercise tolerance and HPA axis hormone of rats.Methods: Randomly divided rats into normal control group,Mingmen moxibustion group,exhaustion control group,Mingmen moxibustion + exhaustion group according to their swimming exhausted time.Rats received a exhaustive swimming after 20 days of intervention.Exhausted time of swimming rats were recorded.The hypothalamus CRH,pituitary ACTH,adrenal gland CORT of rats were detected by biochemical method and compared.Results: The swimming exhausted time of Mingmen moxibustion + exhaustion group were significantly increased compare to exhausted control group(P0.05).Three types of hormone of HPA axis in each group have the same trend: Compared with normal control group,the hypothalamus CRH,pituitary ACTH,adrenal gland CORT of Mingmen moxibustion group had no significant differences.The HPA axis hormone of exhausted control group was significantly increased(P0.01).The HPA axis hormone of Mingmen moxibustion + exhaustion group were significantly lower than that of the exhausted control group(P0.01).Conclusions: Preventive moxibustion at Mingmen(DU-4) could improve the exercise tolerance of rats and prolong their swimming exhausted time.Its physiological mechanism might be related to easing excessive stress of HPA axis on exhaustive exercise.
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