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《Journal of Beijing Medical University》 1999-02
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Effect of Ganoderma lucidum polysaccharides B on TNFα and IFNγ preduction and their mRNA expression

ZHANG Qun-Hao; LIN Zhi-Bin (Department of Pharmacology, Beijing Medical University. Beijing 100083)  
To investigate the effects of Ganoderma lucidum polysaccharides B(GL-B), on production of tumor necrosis factor alpha (TNFα), interferon γ(IFNγ) and their mRNA expression in murine peritoneal macrophage (PM) and splenocytes. Methods: Mouse PM and splenocytes were treated in vitro with GL-B, and cell culture supernatant was separated. Both TNFα and IFNγ were detected by biological assay, and ELISA respectively. The level of mRNA was detected with the method of reverse transcript ion- polymerase chain reaction (RT- PCR ). Results: TNFα production w as dose-dependently increased in the supernatant of normal murine PM by the effect of GL-B 25-400 mg ·L~(-1). The maximal effect was observed after 24 h of incubation and decreased after 72 h. GL-B significantly promoted IFNγ production in normal murine splenocytes with the dose of 12. 5-200 mg ·L~(-1). The maximal effect was observed after 24 h of incubation and decreased after 48 h. GL-B significantly promoted IFNγ mRNA expression in normal murine splenocytes with the dose of 50, 100 and 200 mg· L~(-1). Conclusion: GL-B significantly promoted TNFα, IFNγ mRNA expression in normal murine PM, and splenocytes, thus increased TNFα, IFNγ production.
【Fund】: 中国博士后基金
【CateGory Index】: R285.5
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