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《JOURNAL OF BEIJING MEDICAL UNIVERSITY》 1999-06
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A study on endothelial nitric oxide synthase in pulmonary arteries of hypoxic pulmonary hypertensive rats

CUI Yan Nan, MA Yu Wen, DU Jun Bao (Department of Pediatrics, the First Hospital, Beijing Medical University, Beijing 100034)  
Objective: To study the abundance and localization of nitric oxide synthase (NOS) in pulmonary arteries of rats and to explore the relationship between their changes and chronic hypoxic pulmonary hypertension (HPH). Methods: Immunohistochemistry technique was performed on the lung tissues of the HPH rats with eNOS multiclonal antibody to assay the abundance and localization of eNOS in different segments of pulmonary arteries of the hypoxic and control rats. At the same time, the plasma concentration of nitric oxide (NO) metabolite, nitrite, was measured by spectrophotometry. Results: eNOS expression was found in pulmonary artery endothelial cells and smooth muscle cells, and there was no significant difference among the different segments of pulmonary arteries, whereas the eNOS expression was inhibited in endothelial cells and smooth muscle cells of pulmonary arteries in hypoxia group; The plasma concentration of nitrite in hypoxia group was significantly decreased compared with that of control rats. Conclusion: The results suggest that the decrease in eNOS in pulmonary artery endothelial cells and smooth muscle cells by hypoxia as well as the decrease in plasma concentration of NO may play a role in the development of chronic HPH.
【Fund】: 国家自然科学基金;;卫生部科研基金
【CateGory Index】: R363
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