Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing Medical University》 2000-01
Add to Favorite Get Latest Update

Detection of DNA-methyltransferase activity of leukemia cells with radiology microassay

BAI Zhi Yong 1, XU Guo Bin 2, WU Shu Lan 1 (1. Department of Hematology, 2. Department of Laboratory, the First Hospital, Beijing Medical University, Beijing 100034, China)  
Objective:To establish a sensitive radiology microassay technique for studying activity of DNA methyltransferase in leukemia cells, and evaluate its feasibility. Methods: 1.1×10 8 Bq 3H SAM used as a methyl donor and 0.5 μg Poly[d(I C)d(I C)] as a receptor, MTase activity in mononucleated cells from bone marrow of patients with acute leukemia and of control subjects with no hematological malignancies were detected. cpm were counted with 5 μg cellular protein incubated for 2 hours. All detection was performed in duplicates, each experiment had positive control with HL 60 cells and negative control without Poly [d(I C)d(I C)]. Results: The method was linear up form 700 to 20 000 cpm when the quality of cellular proteins was between 0.25 μg and 7.5 μg, Y cpm = 2 864 X + 142, r =0.998.Within group and day to day variations were 5.9% and 11.4% respectively. The sensitivity of the method was 2.4×10 4 Bq. Mean MTase activity in 15 cases with acute leukemia was (9.1±4.6)%,4 fold higher than that in 11 cases with no hematological malignancies which was ( 2.1± 1.6)% ( P 0.05); and the value from 11 acute leukemia patients in complete remission was ( 4.1± 2.6)%, which was in the same range as the control group. Conclusion:Radiology microassay had high sensitivity and good replication for detecting MTase activity of leukemia cells. MTase activity of patients with acute leukemia increased, and it decreased in thesame range as control group when thecomplete remission was achieved.
【Fund】: 卫生部科学研究基金! ( 94 1 2 5 0 )
【CateGory Index】: Q555
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 ;Epigenetic regulation for prevention and gene therapy of tumors[J];Journal of Xinxiang Medical College;2004-06
2 CHEN Hua,WU Shu-Lan,ZHU Qiang,SHI Yong-Jin,XU Guo-Bin,WANG Jian-Zhong (Department of Hematology,The First Hospital of Peking University,Department of Laboratory,The First Hospital of Peking University,Beijing 100034);Inhibitory Effect on Proliferation of KG1a Cell Line by Methyltransferase Inhibitors[J];Journal of Experimental Hematology;2002-04
3 BAI Zhi-yong, BAI Xiao-chuan, WU Shu-lan * Department of Hematology, the First Teaching Hospital, Peking University, Beijing 100034;RELATIONSHIP BETWEEN THE PATTERN OF METHYLATION OF CALCITONIN GENE AND ACTIVITY OF METHYLTRANSFERASE IN 8 TUMOR CELL LINES[J];中国癌症研究(英文版);2001-03
Chinese Journal Full-text Database 10 Hits
1 Li Ruliu Chen Weiwen Xu Songfen et al Faculty of Traditional Chinese Pharmacology;Comparison of Pharmacology Action of Zuojinwan (ZJW) and Jiawei-ZJW on Digestive Tract[J];;1993-01
2 CHEN Yanfen, CHEN Weiwen , LI Ruliu, YE Fuqiang, XU Songfen, PAN Huaigeng (Pi- Wei Institute, Guangzhou University of TCM, Guangzhou 510405, China);Comparison of Protective Mechanism of Zuojin Pill and Fan Zuojin Pill on Gastric Mucosa[J];Journal of Guangzhou University of Traditional Chinese Medicine;2003-02
3 WANG Xin-gang1,2,WANG Xiao-feng3,LI Fu-nian2,HOU Lin4,YANG Qi-feng1(1.Department of Breast Surgery,Qilu Hospital of Shandong University,Jinan 250012,China; 2.Department of Breast Surgery,Affiliated Hospital of Medical College of Qingdao University, Qingdao 266100,Shandong,China; 3.Department of Medical Microbiology,Medical College,Qingdao University,Qingdao 266021,Shandong,China; 4.Department of Biochemistry and Molecular Biology,Medical College,Qingdao University, Qingdao 266021,Shandong,China);Effects of epigenetic drugs on BRCA1 and CHD5 expressions in breast cancer cells[J];Journal of Shandong University(Health Sciences);2011-07
4 QI Chong,LI Jian-fang,QU Ying,CHEN Xue-hua,LIU Bing-ya,ZHU Zheng-gang(Department of Surgery,Shanghai Institute of Digestive Surgery,Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China);Hypermethylation of multiple tumor-related genes in gastric carcinoma and adjacent normal tissues[J];Journal of Shanghai Jiaotong University(Medical Science);2007-05
5 LU Rong,FANG Jing-Yuan*(Shanghai Institute of Digestive Disease,Shanghai Jiaotong University Medical School,Renji Hospital,Shanghai 200001,China);Epigenetic modification and cancer[J];Chinese Bulletin of Life Sciences;2006-01
6 SUN Kai sheng,XU Ke qian (Department of Laboratory Medicine, College of Medical Technology and Information,Central South University, Changsha 410013,Hunan,China);Histone Acetylation/Deacetylation and Regulation of Gene Expression[J];Life Science Research;2004-S1
7 PAN Langsheng~(1,2),L Xiuyang~1,XU Haidan~1,WU Pingdong~1(1.Institute of Pharmaceutical Engineering,College of Materials Science and Chemical Engineering,Zhejiang University,Hangzhou 310027,China;2.College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China);Study on Content Variation of Chemical Constituents During Combination of Rhizoma Coptidis Alkaloids with Fructus Evodiae Flavonoids by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2006-02
8 He,Zhong-Xiao;Gu, Yu;Rao, Hong;Bai,Jian-Shi; Ma, Qing (Department of Biology, Beijing Normal University, Beijing 100875);The Effect of 5-Aza-2'-deoxycytidine (5-Aza-CdR)on Cell Differentiation and DNA Methylase of HL-60 Cells[J];;1995-04
9 Li Aili 1, 2 Jiang Tao 1 Ma Zhiying 2 Jia Jizeng 1 ( 1 Key Laboratory of Crop Germplasm Resources and Biotechnology Ministry of Agriculture Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences,Beijing 100081; 2 Agricultural University of HeBei, Baoding 071001);Several Methodologies on Improving the PCR Products[J];Biotechnology Information;2003-02
10 DONG Yu_wei,HOU Jin_hui,ZHU Bi_cai,PANG Yong_hong(School of life Science,Xuzhou Normal University,Xuzhou,221116,Jiangsu,China);Concepts related to epigenetics and their advances[J];Journal of Biology;2005-01
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 MIAO Jing,WANG Xue-jing,XU Guo-bin,FAN Qing-feng,DING Jie.Department of Pediatrics,Peking University First Hospital,Beijing 100034,China;Comparison between pyrogallol red-molybdate method and biuret colorimetric assay for determining the total urine protein in puromycin aminonucleoside-treated rats[J];The Journal of Practical Medicine;2009-04
2 ZHANG Dian-hong,CHEN Chuan-gui,HU Shan-shan,MA Zhao,YU Zhen-tao Cancer Institute and Hospital of Tianjin Medical University,Key Laboratory of Prevention and Therapy, Tianjin 300060,P.R.China;Biological significance of 5-Aza-CdR on methylation of BNIP3 gene in human esophageal carcinoma cell line Kyse-140[J];Chinese Journal of Cancer Prevention and Treatment;2013-15
3 LI Yuan *, WU Shu lan, BU Ding fang, ZHU Yan, ZHU Qiang, CAO Xiang hong *Department of Hematology, First Hospital of Peking University, Beijing 100034, China;The expression of DNA methyltransferase DNMT1,3A and 3B in acute leukemia and myelodysplastic syndrome[J];Chinese Journal of Internal Medicine;2003-10
4 LI Yuan, DAI Yue, WU Shu-lan~*, PEI Pei, CAO Xiang-hong, PU Ding-fang. ~*Department of Hematology, First Hospital of Peking University, Beijing 100034, China;The C46359T polymorphism of DNMT3B promoter gene and pathogenesis of acute leukemia[J];Chinese Journal of Internal Medicine;2005-08
5 CHENG Xue-mei,SHAN Bao-de,ZHANG Tian-liang;Environment and Epigenetics[J];Occupation and Health;2010-18
6 WANG Xuan1,LI Dong2,JIANG Cui-min1(1.Department of Pharmacy,Putuo People's Hospital,Shanghai 200060,China;2.Department of Clinical Laboratory,Tongji University affiliated Tongji Hospital,Shanghai 200065,China);Study the effects of CDA-2 on growth and apoptosis of human non-small cell lung cancer cells[J];Chinese Journal of Hospital Pharmacy;2012-17
7 ZHU Hang,LEI Xun,ZHANG Fan,WANG Yang Maternal,Child and Adolescent Health Care Teaching Section,Public Health and Administration School,Chongqing Medical University,Chongqing 400016,China;Risk Factor and Population Attributable Risk of Children Leukemia in China:A Meta-Analysis[J];Chinese Journal of Evidence-Based Medicine;2012-10
8 Hongdian ZHANG;Chuangui CHEN;Zhentao YU;Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin;;Research advancement on BNIP3 in gastrointestinal cancer[J];Chinese Journal of Clinical Oncology;2013-24
9 ZHANG Ming;LIU Zhuo-gang;LI Meng-qi;Department of Hematology,Shengjing Hospital Affiliated to China Medical University;;Correlation between Gene Methylation and the Drug Resistance of Tumor[J];Medical Recapitulate;2014-14
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved