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Hairy root culture and anthraquinone production of Cassia obtusifolia L in a bioreactor

CHANG Zhen Zhan 1, GUO De An 1, ZHENG Jun Hua 1 JIN Ming Shan 2, Xie Mei Yin 2, SHEN Xin 3, WANG Sha Sheng 3 (1.School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083, China; 2. Beijing First School of Light In  
Objective: To explore the new approach for producing secondary metabolites by large scale culture of plant hairy roots. Methods: The hairy roots of Cassia obtusifolia L. were cultured using a bioreactor with capacity of five liters. The pH value and the content of free anthraquinones in the liquid medium were continuously measured. Results: The mass of hairy roots increased by 42 times within the culture period of 36 days and reached 761 g in fresh weight. Conclusion: To some extents, the biosynthesis of free anthraquinones in the bioreactor was reflected by the variation of pH values in the culture medium. (J Beijing Med Univ, 2000,32:142 144)
【Fund】: 国家自然科学基金!(39470 849、39770 90 1 )
【CateGory Index】: R914
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