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《JOURNAL OF BEIJING MEDICAL UNIVERSITY》 2000-05
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A study on the correlation between the expression of matrix metalloproteinase 9 and the metastatic phenotype in human melanoma cells

LI Hong Mei 1, FANG Wei Gang 1, XIE Jian Wu 2, ZHENG Jie 1, YOU Jiang Feng 1, WU Bing Quan 1 (1. Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China; 2. National Institute of Health, USA)  
Objective: To investigate the correlation between MMP 9 and tumor metastasis. Methods: A mammalian expression vector containing antisense cDNA of MMP 9 was constructed and transfected into a metastatic human melanoma cell line WM451 which had a high MMP 9 expression level. In vitro and in vivo tests such as MTT [3 (4,5 dimethylthiazol zyl) 2,5 diphenyl tetrazolium bromide] method, zymography as well as xenografting in nude mice experiments were used to analyse expression of MMPs and in vitro and in vivo behaviors of tumor cells before and after gene transfection. Results: After transfection the expression and activity of MMP 9 decreased significantly. The production of MMP 2 was also suppressed. The capacity of in vitro growth, invasion through Matrigel, tumorigenecity as well as spontaneous metastasis in nude mice was reduced to some extent. Conclusion: The decrease of MMP 9 expression in human melanoma cell line by MMP 9 antisense RNA could inhibit its metastatic phenotype and demonstrated that MMP 9 might play a central role in metastasis.
【Key Words】： Melanoma/enzymol Neoplasm metastasis Metalloproteinases/metab RNA antisense
【Fund】： 国家自然科学基金!(39870 35 7)
【CateGory Index】： R739.5
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