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Inhibitory effect of IFN-γ transgene expression on allergen induced murine pulmonary eosinophil infiltration

GAO Zhan Cheng 1, KANG Yu 1, SHANG Ying 1, GAI Jun 1, YU You Zhi 2, HE Quan Ying 1 (1. Department of Respiratory Medicine, 2. Department of Pathology, Peking University People's Hospital, Beijng 100044, China)  
Objective: To investigate the effect of IFN γ transgene expression on allergen induced pulmonary eosinophil infiltration in murine asthmatic model. Methods: C57 mice were primed intraperitoneally and challenged by aerosol with ovalbumin (OVA) to produce asthmatic model. Bronchoalveolar lavage (BAL) and lungs were recovered after 48 hour OVA post challenge. Replication deficient adenovirus with IFN γ gene (AdCMVIFNγ) 5×10 8 plaque forming unit (pfu) was administrated intratracheally in murine asthmatic model 48 hours before OVA challenging. BAL and lungs were recovered after 48 hour OVA post challenge. Results: In asthmatic model, eosinophil infiltrated around airways and blood vessels, and partial alveolar sacs histologically, but non OVA sensitized mice showed none of these changes. The eosinophils (Eos) constituted an average of (75.13± 6.85)% in BAL, whereas no Eos were found in BAL in the negative control ( P 0.001). In AdCMVIFNγ treated asthmatic models, histologic evaluation revealed marked suppression of eosinophil peribronchial and perivascular infiltration; the recoverable Eos in BAL were an average of (9.00±4.58)%, which was statistically decreased, compared with the asthmatic control group ( P 0.001). Conclusion: IFN γ transgene expression via adenovial vector in pulmonary epithelia in vivo abrogate allergen induced eosinophilic infiltration in lungs in asthmatic model, which may provide a new therapeutic method for allergic asthma with cytokine gene transfer.
【Fund】: 国家自然科学基金!(3980 0 0 6 7)
【CateGory Index】: R562.2
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