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《Journal of Beijing Medical University》 2001-01
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DNA demethylation leads to telomere shortening with accelerated aging process in human diploid fibroblasts

CHEN Pei Li, TONG Tan Jun, ZHANG Zong Yu (Department of Biochemistry and Molecular Biology, Peking University School of Basic Medical Sciences, Beijing 100083, China)  
Objective: The effect of DNA demethylation on aging process and telomere length was studied. Methods: The effect of 5 aza cytidine treatment on aging process of human fetal lung diploid fibroblasts (2BS) was determined by cellular morphology and telomere length assayed by Southern blotting. Results: The results showed that DNA demethylation could advance the senescent phenotype more significantly in old cells than it did in young cells. Accordingly, telomere shortening seemed to be more apparent in old cells (about 96% of that of control). Conclusion: The decreased level of DNA methylation and the resulted telomere shortening may be important cooperative factors contributing to cellular aging process.
【Fund】: 国家自然科学基金重点项目! ( 39930 170 )&&
【CateGory Index】: R346
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Zong-Yu;Fan Xin-Qing;Tong Tan-Jun(Department of Biochemistry and Molecular Biology,Beijing Medical University,Beijing 100083);Change of Telomere Length of Human Embryo Lung Diploid Fibroblasts(2BS)at Different Population Doublings[J];;1997-01
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Yingfang,TIAN Xinxia,DING Xin (Inner Mongolia University of Science and Technology, Baotou 014010,China);Methylation of Gene E-cadherin 5’CpG Island in Gastric Carcinomas[J];Journal of Baotou Medical College;2005-01
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