Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing Medical University》 2001-03
Add to Favorite Get Latest Update

Campneoside Ⅱ of Cistanche tubulosa (Schenk) R. Wight protects neurons from apoptosis induced by neurotoxin 1 methyl 4 phenylpyridinium (MPP +)

PU Xiao Ping 1, LI Xiao Rong 1, LI Hui Nong 1, TU Peng Fei 2, SONG Zhi Hong 2, LI Chang Ling 1 (1. Department of Molecular and Cellular Pharmacology, 2. Department of Natural Medicines, Peking University School of Pharmaceutical Sciences, B  
To establish an apoptosis model induced by 1 methyl 4 phenylpyridinium ion (MPP +) in Cerebellar granule neurons (CGNs) and assessed the effect of campneoside Ⅱ which is a compound contained in Cistanche tubulosa (Schenk) R. Wight. Methods: CGNs were treated with MPP + and then staining with methyl green and pyronin, internucleosomal DNA degradation with agarose gel electrophoresis analysis and flow cytometry of neuronal apoptosis were performed . Results: Treatment of 50 μmol·L -1 MPP + for 24 h or longer induced an initiated apoptosis in CGNs and the latter showed typical morphological features, including condensed chromatin, nuclear fragmentation and apoptotic body. Agarose gel electrophoresis of DNA from the cells treated with MPP + revealed “ladder” pattern and a time dependent increase of apoptotic peak by flowcytometric analysis. Treatment of 25 mg·L -1 campneoside Ⅱ could protect cells from apoptosis induced by MPP +. The degraded fragments of DNA decreased obviously. The viability percentage of cells was improved within 24 h. Conclusion: A new and simple method of quantitating MPP + induced apoptosis in CGNs was established. The campneoside Ⅱ inhibited MPP + induced apoptosis and the DNA fragmentation. This method can be used for studying apoptosis mechanism involved in the pathogenesis of Parkinson disease and screening anti Parkinson's disease drugs.
【Fund】: 国家重点基础性研究 973项目基金!(G19980 5 112 4)资助&&
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yu-zhe,YANG Ling-ling,SONG Hai-feng,DU Yi-min (1.Pharmaceutical Institute of Dali University,Dali 671000,China;2.Key Pharmacokinetics Laboratory,Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China);Anti-stress pharmacological action of Cistanche deserticola and the mechanism:research advances[J];Journal of International Pharmaceutical Research;2009-05
2 TANG Xiao-lei,SUN Ping-fei Zhejiang Pharmaceutical College,Ningbo,Zhejiang,China,315100;Study Progress of C.tubulosa(Schenk)R Wight[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2009-05
3 Zhang Lin, Liu Shu-minInstitute of Traditional Chinese Medicine, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang Province, China;Recent developments of traditional Chinese medicine in treatment of Parkinson disease[J];Chinese Journal of Clinical Rehabilitation;2006-03
4 YAN Yu,DING Qi-long(Department of Physiology,China Pharmaceutical University,Nanjing 210009,China);Advances in the Mechanistic Study on Neuroprotective Actions of the Monomers and Active Components from Chinese Traditional Medicines[J];Progress in Pharmaceutical Sciences;2007-04
5 WU Bin,ZHAO Shuzhi,WANG Xiumin,DONG Qiqian,ZHENG Guoqing*(Department of Neurology,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China);Mechanism of traditional Chinese medicine on animal model of parkinson′s disease[J];China Journal of Chinese Materia Medica;2011-18
6 XIONG Pei,CHEN Xin,ZHANG Nan(Traditional Chinese Medical School,Capital Medical University,Beijing 100069,China);Advance in studies on pathological mechanism of Parkinson′s disease and traditional Chinese medicine experiments in prevention and treatment of Parkinson′s disease[J];China Journal of Chinese Materia Medica;2012-05
7 Meng Gen Wei Kaihua National Center of Biomedical Analysis,Beijing,100850;Progress in Active Components and Their Pharmacology of Several Specific Tradtional Mongolia Herbal Drugs[J];Journal of Medicine & Pharmacy of Chinese Minorities;2004-01
8 BAO Xiao-dong,ZHAO Jin-ping.The Chinese Medicine University of Zhejiang,Hangzhou 310053,China;The Research on the Protective Effect of Ping-pa Decoction on Dopaminergic Neurons in PD Mouse Models[J];Medical Innovation of China;2012-16
9 TIAN Jiyu1, CHEN Jianzong1, CHEN Xiaoli2(1.Research Center of TCM,XiJing hospital,Fourth Military Medical University,Xi'an 710032;2.Dept.of Pharmacy,XiJing hospital,Fourth Military Medical University, Xi'an 710032);Protective effect of Prescription for Nourishing Liver- Kidney on Injured Cultured PC12 Cell in Vitro[J];Traditional Chinese Drug Research & Clinical Pharmacology;2004-01
10 Zhou Yixian, Bao Yuancheng Wanwei Hospital, Chaohu, Anhui (Chaohu 238002);Clinical Research on Kangzhenzhijing Capsule Ⅱ with Madopar for Parkinson’s Disease[J];Chinese Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease;2007-04
China Proceedings of conference Full-text Database 2 Hits
1 ;醉鱼草苯乙醇苷成分抗中脑神经细胞凋亡机制的研究[A];[C];2002
2 TIAN Jiyu1, CHEN Jianzong1, CHEN Xiaoli2(1.Research Center of TCM,XiJing hospital,Fourth Military Medical University,Xi'an 710032;2.Dept.of Pharmacy,XiJing hospital,Fourth Military Medical University, Xi'an 710032);Protective effect of Prescription for Nourishing Liver- Kidney on Injured Cultured PC12 Cell in Vitro[A];[C];2004
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Cong-Mei1, HUANG Tian-Hua1,, WU De-Sheng, et al( 1. Research Center of Reproductive Medicine, Shantou University Medica College, Shantou 515031, China; 2. Department of forensic medicine, Shantou University Medical College, Shantou 515031, China );CONSTRUCTION OF pEgr-P16 PLASMID AND ITS EXPRESSION IN EC9706 CELL INDUCED BY IONIZING IRRADIATION[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-04
2 RUAN Ye 1 ,WANG Xiao-yun 1 ,XIE Qing-dong 2 ,et al (1.School of Public Health,Lanzhou Medical College,Lanzhou730000,China;2.Research Center for Reproductive Medicne,Shantou University Medical College,Shantou515031,China);Experimental Study on Sperm-mediated Foreign DNA Transfer[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-03
3 OUYANG Song_ying 1 ,XU Shou_shui 2, ,SHEN Wen_lu 2 ,et al (1.Faculty of Military Veterinary Medicine,The Quartermaster University of PLA,Changchun130062,China;2.The Second Affiliated Hospital of Shantou University Medical College,Shantou515041,China);Construction of PTEN Gene Expression Vector and Its Expression in Glioma Cell Lines U_(251)[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-04
4 CHEN Chuan-de, WU Zhong-liang, CHEN Jia-kun(Center for Disease Control and Prevention of Luohu district,Shenzhen, Shenzhen 518020, China; Institude forChemical Carcinogenesis, Guangzhou Medical College,Guangzhou 510182, China);Genomic Instability in Transformed 16HBE Cells Induced by Nickel Acetate[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-01
5 XIONG Xiao-fang,HUANG Tian-hua ˇ ,XIE Qing-dong,WANG Xiao-mei,CHEN Gui-lan (Research Center for Reproductive Medicine, Shantou University Medical College,Shantou515041,Guangdong,China);Replication and Expression of Sperm-mediated HBV DNA in Early Mouse Embryo[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-03
6 ZHU Qi-xing1,2, MA Tai2, TU Deng-yun2, SHEN Tong2, DING Rui2(1.The First Affiliated Hospital of Anhui Medical University,Hefei 230031,Anhui,China; 2.Central Laboratory of Toxicology,Public Health School, Anhui Medical University, Hefei 230032,Anhui,China);Effect of Ginkgo Biloba Extract on Trichloroethylene-induced Nitric Oxide Synthesis in Human Keratinocytes[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-06
7 Liu Jiaren 1, Pang Yongxun 1, Zhang youen 1, Wang Xianzhen 1, Fan Chun 1, Zhu Zhengang 1, E.J.Love 2 1Department of Environmental Health of Harbin Medical University, Harbin\ 150001, 2 Department of Community Health Sciences, Faculty of Medicine, The;EVALUATION ON CARCINOGENIC RISK FROM ORGANIC POLLUTION IN THE S RIVER BY CELL TRANSFORMATION[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1999-04
8 LüHui-zhen,ZHANG Jian-Peng,CHEN Xin,LIU Jing,ZHANG Yuan-hu(College of Life Science,Shandong Agricultural University,Shandong Key Laboratory of Crop Biology,Taian 271018,China);A Modified Method for RNA Purification from Yali Pear(Pyrus bretschneideri Rehd.)[J];Shandong Agricultural Sciences;2007-05
9 ZHANG Xiu-hua,WANG Wen-feng(Department of Life Science and Technology,Xinxiang Medical University,Xinxiang 453003,China);Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA[J];Shandong Agricultural Sciences;2009-06
10 ZHANG Xue-min,WANG Jin-sheng (Key Lab. of Monitory and Management of Plant Diseases and Insects, Ministry of Agriculture of Nanjing Agric. Univ.,Nanjing 210095,China);A Modified Alkaline Lysis Method for the Preparation of Plasmid DNA[J];Journal of Anhui University(Natural Sciences);2000-04
China Proceedings of conference Full-text Database 10 Hits
1 Jun Liu weijia kong Rongxiang Zhao;Origination of progenitor cells of mammalian regenerative hair cells[A];[C];2009
2 Feng Ting,Tu Wenwei,Huang Juan,Li Jingrong,Yin Zhongwei,Fan Yingying,Li Hong.The Joint Research Center of West China Second University Hospital of Sichuan University and Faculty of Medicine,the University of Hong Kong, Chengdu 610041,China;Optimization of Culture condition for cultivation of influenza virus H1N1 on MDCK cell and purification[A];[C];2011
3 ;子房注射法获得转基因甜瓜植株[A];[C];2006
4 LI Junhua~2,WU Shaoting~1,WENG Yabiao~2,Gan Yan~1,Liu Hongbo~3,Liu Maoling~3, Zhang Renli~1,GAO Shitong~1,Huang Dana~1,Geng Yijie~1 (1.Shenzhen Center for Disease Control and Prevention,Shenzhen 518020 2.college of veterinary medicine South China Agricultural University,Guangzhou 510642 3 Tongji Medical College,Hua Zhong University of Science and Technology,Wuhan,430030);In vitro Amplification,cloning of the SAG2 gene of Toxoplasmagondii and its Exprcsson in Mycobacterium smegmatis mc2 155[A];[C];2006
5 LI Junhua~2,WU Shaoting~1,WENG Yabiao~2,Gan Yan~1,Liu Hongbo~3,Liu Maoling~3, Zhang Renli~1,GAO Shitong~1,Huang Dana~1,Geng Yijie~1 (1.Shenzhen Center for Disease Control and Prevention,Shenzhen 518020 2.college of veterinary medicine South China Agricultural University,Guangzhou 510642 3 Tongji Medical College,Hua Zhong University of Science and Technology,Wuhan,430030);In vitro Amplification,cloning of the GRA4 gene of Toxoplasma gondii and its Expresson in Mycobacterium Ssmegmatis mc2 155[A];[C];2006
6 HUANG Jiangxun,MA Zheng TANG Xiaojiang,et al.(Guangdong Provincial Center for Occupational Disease Prevention and Treatment, Guangzhou,510300,China);Research on n-Hexane Mediated Cross-Linking of Erythrocyte Spectrin[A];[C];2004
7 Yan Jiabin Li Xinhui Wu Shuqin Du Hejun;The expression for specific nucleic acid of Siniperca chuatsi virus and the preparation for high efficient antibody[A];[C];2003
8 HU Jiao TAN Xiao-feng ZHANG Lin LONG Hong-xu TIAN Xiao-ming Hunan Changsha,The Key Lab of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,410004;Improvement of methods for total RNA isolation from seeds of Camellia oleifera[A];[C];2009
9 WANG Ze-Sheng CHEN Mei-Yuan LIAO Jian-Hua LU Zheng-Hui GUO Zhong-Jie LI Hong-Rong (Fujian Research Institute of Light Industry, Fuzhou, 350005,P.R.China);THE FULL-LENGTH CDNA SEQUENCE OF A GENE RELATED TO THE THERMOTOLERANCE OF AGARICVS BISPORUS[A];[C];2005
10 LU Luo, YING Kang, WEI Shaoming, LIN Huifen,MA Laiji,MAO Yumin Research & Development Department of Shanghai Jahwa Co. LTD., Shanghai 200082. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433.;The induced activity of asiaticoside for the dermal fibroblasts in collagen synthesis[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Yu-xia et al(College of Life Science,Shihezi University,Shihezi,Xinjiang 832003);Determination of Amino Acids in Cistanche deserticols Ma and its Nutritional Evaluation[J];Journal of Anhui Agricultural Sciences;2007-17
2 Bao Yuancheng, Zhou Houguang, Wang Han, et al The Neurology Department,Affiliated Hospital,Anhui College of TCM, Hefei 230038;The experimental study on treatment of kangzhen Zhijing capsule combined with Glutathione in the model rats with Parkinson disease[J];Anhui Medical Journal;2002-05
3 BAO Yuan-cheng,ZHOU Hou-guang,WANG Han,ZHANG Bo,CHEN Huai-zhen, YANG Xing-tao,WANG Yan-xin,WANG Xiao-yang,XU Guo-cun (The Dept. of Neurology,the First Affiliated Hospital, Anhui College of TCM,Anhui Hefei 230031,China);EFFECT OF KANGZHEN ZHIJING CAPSULE COMBINED WITH GLUTATHIONE ON BLOOD ANTIOXIDASE IN PARKINSON DISEASE PATIENTS[J];Journal of Anhui Traditional Chinese Medical College;2001-04
4 LI Gang,CHEN Jian zong,LIANG Hong juan,ZHANG He long,JI Tong li (Department of Oncology,Tangdu Hospital,the Fourth Military Medical University,PLA,Shaanxi Xi′an 710032,China);Effect of Peibu Ganshen Recipe on Changes of Behavior and Nigra Monoamine Transmitters of Brain in Mice with Parkinson′s Disease[J];Journal of Anhui Traditional Chinese Medical College;2003-06
5 CHENJian-zong,LIGang,LIANG Hong-juan(Institute ofTraditional Chinese Medicine,Xijing Hospital,theFourth Military Medical University,Shaanxi Xi′an 710032,China);Effect of Peibu Ganshen Recipe on Changes of Monoamine Transmitters of Brain in Mice of Parkinson′s Disease Model[J];Journal of Anhui Traditional Chinese Medical College;2005-05
6 Li Baiqing, (Department of Microbiology, Bengbu Medical College, Anhui );EFFECTS OF CHINESE DRUGS INVIGORATING KIDNEY ON CELLULAR IMMUNITY I . Effect of Epimedium and Cistanche on E-rosette formation and activity of acid α——naphthyl acetate esterase in human lymphocyte[J];Journal of Bengbu Medical College;1985-03
7 XU Fang1,RUI Yu-kui2(1.Biochemical Engineering College of Beijing Union University,Beijing 100023,China;2.College of Resources and Environmental Science,China Agricultural University,Beijing 100094,China);Contents of Ten Trace Elements in Wild Cistanche deserticola Y. C. Ma[J];Northern Horticulture;2008-12
8 Wang Miqu (Chengdu University of TCM, Chengdu 610075)Zeng Xiangguo(Huaxi Medical University)Lin Qiao (The Secondary Institute of Agriculture and Animal Husbandry in Chengdu);Priliminary Report on Testicular Pathologic Pictures in Rats Suffering Congenitle Kidney Dificiency[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;1998-02
9 Feng Wei,Shi Yinyu,Shen Peizhi,etal Orthopedics Instituteof Shanghai University of TCM (2 0 0 0 3 2 );ln Vitro Study of Sera Contained in Bushenfang and Rouganfang (China herbs) on Proliferation Dynamics of Chondrocytes[J];Journal of Zhejiang College of Tcm;2001-04
10 Gong Bin, Yang Wengang, Zhang Qian et al. (Shanghai University of Traditional Chinese Medicine, Shanghai 200032);Effect of Chinese Kidney-tonifing Herbal Medicine on Function of M Receptor in Brain Tissue of Aged Rats[J];LABELED IMMUNOASSAYS AND CLINICAL MEDICINE;1999-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Li Fuxiang(Qinghai Normal university biotechnology department 810008);Current Status and Progress of Pharmacological Research in Cistanche[J];Anhui Agricultural Science Bulletin;2006-13
2 WANG Xiao-qin et al (Department of Life Science and Engineering,Hexi University,Zhangye,Gansu 734000);Study on the Extraction Technology and Antimicrobial Action of Cistanche deserticola Polysaccharides[J];Journal of Anhui Agricultural Sciences;2009-32
3 GAO Zhan-you et al(Tianjin Zhongrui Pharmaceutical Co.,Ltd,Tianjin 301712);Effects of HERBA CISTANCHES on the Ability of Resistance Exercise-induced Fatigue in Rats and Free Radical in Brain Tissue[J];Journal of Anhui Agricultural Sciences;2011-16
4 Ai Zongyao,Lu Huirong Huzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University(313000);Application of TCM Cream Formula in Parkinson Disease[J];Journal of Zhejiang Chinese Medical University;2012-06
5 LI Da-xiang,XIAN Shu,YANG Wei,WAN Xiao-chun State Key Laboratory Cultivation Base of Tea Biochemistry & Biotechnology,Anhui Agricultural University,Hefei 230036,China;Progress on the Neuroprotective Effects of Tea[J];Journal of Tea Science;2011-02
6 HAO Yuan-yuan1,2,YUE Li-jun1,KANG Jian-jun1,WANG Suo-min1 (1.State Key Laboratory of Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;2.School of Life Sciences,Lanzhou University,Lanzhou 730000,China);Research progress on "desert ginseng"-Cistanche deserticola and Cynomeorium songaricum[J];Acta Prataculturae Sinica;2012-02
7 HU Ying1,TAN Qijian2(1.School of Medicine and Nursing,Chengdu University,Chengdu 610106,China;2.Department of Mathematics,Chengdu Normal University,Chengdu 611130,China);Mathematical Models of Chinese Medicinal Resources in Desert and Oasis Area[J];Journal of Chengdu University(Natural Science Edition);2013-02
8 WEN Xiao-dong;REN Ding;WANG Chun-ling;KONG De-yan;WANG Kai-hua;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine;Guangxi University of Chinese Medicine;;Effects of Chinese herbal medicine Pabing Ⅱ Formula on quality of life of patients with Parkinson's disease at early-stage[J];China Journal of Traditional Chinese Medicine and Pharmacy;2013-10
9 CHENG Qi-lai,LI Hong-liang,DING Ye-chun(Department of Pharmaceutical,Gannan Medical University,Ganzhou,341000,Jiangxi,China);Determination of Trace Metal Elements in Cistanche deserticola by Atomic Absorption Spectrometry[J];Hubei Agricultural Sciences;2009-01
10 HE Xin1,SUN Hong-mei1,WU Hai-xia1,XU Hong1,WANG Yuan-yuan1,GAO Yu-shan1,WANG Zhi-yong1,GE Gui-ling2,BAI Li-min1 ( 1. Department of Anatomy,Beijing University of Chinese Medicine,Beijing 100029,China; 2. Development Center of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China);Neuroprotective Effect of Dabuyin Pill,Qianzheng powder and Combined Formula on Parkinson's Disease Mice Brain[J];Liaoning Journal of Traditional Chinese Medicine;2010-11
China Proceedings of conference Full-text Database 1 Hits
1 ZHOU Xiu-juan JU AI-hua ZHANG Hui-wen(Inner Mongolia Medical College,Hohehot 010059);Research Developement about Medicinal Plant resources and chemical constituents of Orobanchaceae[A];[C];2007
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved