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《Journal of Beijing Medical University》 2001-03
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Campneoside Ⅱ of Cistanche tubulosa (Schenk) R. Wight protects neurons from apoptosis induced by neurotoxin 1 methyl 4 phenylpyridinium (MPP +)

PU Xiao Ping 1, LI Xiao Rong 1, LI Hui Nong 1, TU Peng Fei 2, SONG Zhi Hong 2, LI Chang Ling 1 (1. Department of Molecular and Cellular Pharmacology, 2. Department of Natural Medicines, Peking University School of Pharmaceutical Sciences, B  
To establish an apoptosis model induced by 1 methyl 4 phenylpyridinium ion (MPP +) in Cerebellar granule neurons (CGNs) and assessed the effect of campneoside Ⅱ which is a compound contained in Cistanche tubulosa (Schenk) R. Wight. Methods: CGNs were treated with MPP + and then staining with methyl green and pyronin, internucleosomal DNA degradation with agarose gel electrophoresis analysis and flow cytometry of neuronal apoptosis were performed . Results: Treatment of 50 μmol·L -1 MPP + for 24 h or longer induced an initiated apoptosis in CGNs and the latter showed typical morphological features, including condensed chromatin, nuclear fragmentation and apoptotic body. Agarose gel electrophoresis of DNA from the cells treated with MPP + revealed “ladder” pattern and a time dependent increase of apoptotic peak by flowcytometric analysis. Treatment of 25 mg·L -1 campneoside Ⅱ could protect cells from apoptosis induced by MPP +. The degraded fragments of DNA decreased obviously. The viability percentage of cells was improved within 24 h. Conclusion: A new and simple method of quantitating MPP + induced apoptosis in CGNs was established. The campneoside Ⅱ inhibited MPP + induced apoptosis and the DNA fragmentation. This method can be used for studying apoptosis mechanism involved in the pathogenesis of Parkinson disease and screening anti Parkinson's disease drugs.
【Fund】: 国家重点基础性研究 973项目基金!(G19980 5 112 4)资助&&
【CateGory Index】: R285.5
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