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《Journal of Beijing Medical University》 2002-03
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Influence by nitric oxide on expression of HO-1 mRNA in pulmonary artery smooth muscle cells

TIAN Hong 1, DU Jun Bao 1△ , ZHAO Wei Hong 2, TANG Chao Shu 3 (1.Department of Pediatrics, 2. Maternal and Child Health Center, 3. Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China )  
SUMMARY Objective: To study the influence on expression of HO 1 mRNA and production of carbon monoxide (CO) in rat pulmonary artery vascular smooth muscle cells (PASMCs) by NO donor Sodium nitroprusside (SNP), in order to demonstrate a regulating role in heme oxygenase/carbon monoxide system (HO/CO) by NO. Methods:PASMCs of Wistar rat were cultured in vitro, incubated with SNP (10 -3 mol·L -1 and 10 -4 mol·L -1 ) for 12 h or 24 h under normoxic and hypoxic conditions, respectively. The expression of PASMC HO 1 mRNA was measured using fluorescent real time quantitative RT PCR and the relative amounts of CO and NO released into medium by PASMCs was detected using spectrophotometry. Results: Hypoxia induced the expression of HO 1 mRNA in rat PASMCs, associated with an increase in HO activity as measured by CO production. Treatment of PASMC with NO donor SNP stimulated HO 1mRNA and CO production in a concentration and time dependent tendency both in normoxic and hypoxic conditions. Conclusion:Exogenously administered NO stimulated HO 1 gene expression and CO production in rat PASMCs under nomoxia and hypoxia, upregulating the activity of HO/CO system.
【Fund】: 国家自然科学基金 (3 9870 844 ;3 0 0 70 796);; 国家重大基础研究发展规划项目 (G2 0 0 0 0 5 690 5 ) ;; 北京市自然科学基金项目(70 12 0 2 1)资助~~
【CateGory Index】: R363
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