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《Journal of Beijing Medical University》 2002-03
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Changes of calcitonin gene-related peptide in spinal cord and acetylcholine receptor in skeletal muscle after upper motoneuron injury in rats

YING Shun Wei △, WU Xin Min, WANG Geng (Department of Anesthesiology, Peking University First Hospital, Beijing 100034,China)  
SUMMARY Objective: To observe the changes of calcitonin gene related peptide (CGRP) in skeletal muscle and CGRP mRNA in spinal cord after upper motoneuron injury in rats. Methods:Twenty four adult Wistar rats were randomly divided into four groups. Spinal cord transection in lower thoracic segment was manipulated after anesthesia. The spinal cord beneath the transection level and bilateral soleus muscle were removed on the day of the surgery(control), 1st, 4th and 7th days after the surgery, respectively. Soleus muscle was sectioned in a cryostat microtome into vertical 20 μm sections. Acetylcholine esterase of soleus muscle sections was detected by Karnovsky Roots direct staining. CGRP of soleus muscle sections were detected by immunohistochemistry. The level of CGRP mRNA of spinal cord was determined by RT PCR. The level of AChR subunit mRNA was detected by Northern blot. Results: The distribution of CGRP in neuromuscular junction was the same as that of acetylcholine esterase. The levels of CGRP in soleus muscle sections were significantly decreased from four days after surgery, and the levels of CGRP in soleus muscle sections were markedly decreased on the seventh day after surgery. The levels of CGRP mRNA were significantly decreased from one day after surgery. The levels of γ and ε subunits mRNA of AChR significantly increased. Conclusion: The △Corresponding author email,yingshw@hotmail.com levels of CGRP of soleus muscle and the levels of CGRP mRNA of spinal cord were significantly decreased in parallel after upper motoneuron injury. CGRP may play a role in regulating the acetylcholine receptors at the neuromuscular junctions of target muscle after upper motoneuron injury in rats.
【CateGory Index】: R614.2
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