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《Journal of Beijing Medical University》 2003-02
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Effects of lanthanum on alkaline phosphatase

HU Jian, JIA Yu, LIU Xiang Tao △, LI Rong Chang, WANG Kui (Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, Beijing 100083,China)  
Objective: To investigate the effects of lanthanum on the activities of two kinds of alkaline phosphatase (AP) rat liver AP and bovine intestine AP,and the preliminary mechanism involved. Methods: A traditional colorimetric method was used to measure the activity of AP. The influence of La 3+ on the intrinsic fluorescence of protein was studied by the method of fluorescence titration into the AP solution by LaCl 3. The quenching equation was fitted based on the fluorescence spectrum, and then the binding number and the binding constants were calculated. Results: Lanthanum increasesd the activity of rat liver AP, whereas it decreased that of bovine intestine AP. It was observed that the binding of La 3+ quenched the fluorescence of bovine intestine AP, which indicated that La 3+ could change the conformation of AP in solution. The number of binding sites and the binding constants that were calculated based on the fitted quenching equation were 78 and 2.1×10 4 respectively. Conclusion: La 3+ has different effects on the activities of rat liver AP and bovine intestine AP; the binding of La 3+ can induce the changes of conformation of bovine intestine AP, and inhibit its activity.
【Fund】: 国家自然科学基金 (2 9890 2 80 )~~
【CateGory Index】: R341
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Chinese Journal Full-text Database 2 Hits
1 ZHOU Chong-song, LAN Chang-yun, FAN Bi-wei, LIU Wen-hong (College of the Engineering of Material and Biology, Chengdu University of Technology, Chengdu 610059, China);Functional mechanism of metallic ions in the activity of life[J];Guangzhou Chemistry;2005-01
2 WANG Xiao-ping1, HU Lin-feng1, LIN Juan1, YU You-cheng2, JIANG Xin-quan3, ZHANG Xiu-li3, ZHOU Zeng-tong3, SHI Hong-jun4 (1. Department of Conservative Dentistry, School of Stomatology, Tongji University, Shanghai 200072; 2. Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai 200324; 3. Research Institute of Stomatology, 9th People's Hospital, College of Medicine, Shanghai Jiaotong University, Shanghai 200011; 4. School of Medicine, Tongji University, Shanghai 200092, China);Effect of LaCl_3 on Proliferation of Dog's Bone Marrow Stromal Cells Cultured in vitro[J];Journal of Oral and Maxillofacial Surgery;2007-01
Chinese Journal Full-text Database 1 Hits
1 YANG Wei dong 1,WANG Ting 3,LEI Heng yi 2,LIU Jie sheng 1,YANG Yan sheng 2 (1.College of Life Sciences and Biotechnology,Jinan University,Guangzhou 510632; 2.College of Chemistry and Chemical Engineering,Zhongshan University, Guangzhou 510275;Progress in Studies on Biological Effects of Rare Earth[J];CHINESE RARE EARTHS;2000-03
Chinese Journal Full-text Database 10 Hits
1 XU Xiang-gui et al (Department of Biology,Suzhou Science and Technology College,Suzhou,Jiangsu 215009);Effect of Lanthanum on Activity of Isolated Rat's Ileum[J];Journal of Anhui Agricultural Sciences;2006-12
2 YE Ya-xin et al(Department of Biology,Suzhou University of Science and Technology,Suzhou,Jiangsu 215009);Effects of Lanthanum on the Protective Enzyme System in Radish Seedlings under Low Temperature Stress[J];Journal of Anhui Agricultural Sciences;2008-09
3 SHANG Yan-fang et al(School of Chemistry and Chemical Engineering,Nantong University,Nantong,Jiangsu 226000);Study on Eu~(3+) on Channels in Radish (Raphanus satirus L.) Vacuoles by Patch-clamp[J];Journal of Anhui Agricultural Sciences;2008-17
4 CAO Yong et al(Department of Chemistry,Guangxi Teachers University,Nanning,Guangxi 530001);Effects of La and La(POA)_3 on Physiological Activity of Mung Bean Seedings Stressed by Cd[J];Journal of Anhui Agricultural Sciences;2008-30
5 GUO Shu-hua(Bioengineering School of Weifang University,Weifang,Shandong 261061);Effects of Rare-earth Element Samarium on Pollywog's Growth and Development[J];Journal of Anhui Agricultural Sciences;2009-26
6 ZHUO Yan et al(Chemical and Biological Engineering College,Suzhou University of Science and Technology,Suzhou,Jiangsu 215009);Protective Effect of La~(3+) on Radish Seedlings under Cd~(2+) Stress[J];Journal of Anhui Agricultural Sciences;2009-31
7 JIANG Cheng-yan et al(School of Life Sciences and Technology,Honghe College,Mengzi,Yunnan 661100);Study on the Effect of the Polysaccharide from Pu-erh Tea on the Anti-fatigue[J];Journal of Anhui Agricultural Sciences;2012-01
8 GAO Hong-jian~(1,2),CHANG Jiang~2,ZHANG Zi-li~2,JIANG Xin~1(1.Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008;2.Department of Soil and Agrochemistry,Anhui Agricultural University,Hefei 230036);Effects of La~(3+) on Contents of Amino Acid and Organic Acidin Root Exudates of Rice (Oryza sativa L.)[J];Journal of Anhui Agricultural University;2004-01
9 WANG Ling1, HONG Fa-shui2(1.Library, Suzhou Vniversity, Suzhou 215006, China;2.Colegge of Life Science, Suzhou University, Suzhou 215006, China);Progress of Study on Rare Earth Elements on Chlorophyll Formation[J];Journal of Anhui Technical Teachers College;2004-01
10 Nie Yuxiu,Cui Yanan ,Huang Kexin,et al (The School of Basic Medical Sciences; Third Clinical Collgeg);Histochemical and electromicroscopic observation on effects of low dose chlorides of lanthanum and ytterbium on rat liver[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-02
China Proceedings of conference Full-text Database 2 Hits
1 YU Tai-bao,XU Wei-bing,ZHOU Zheng-fa,REN Feng-mei (Department of Polymer Science and Engineering,Hefei University of Technology,Hefei 230009,China);Synthesis and thermochemical,fluorescence,antibacterial properties of new ternary lanthanum complex La(cin)_2(imi)_2·H_2O[A];[C];2010
2 FENG Jiang-Hua LI Xiao-Jing PEI Feng-Kui (Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,130022);~1H NMR Studies of Biofluids on Long-term Toxicity of Rare Earth[A];[C];2000
Chinese Journal Full-text Database 10 Hits
1 WANG Xiuli \ Department of Biochemistry and Molecular Biology,Qingdao University Medical College,Qingdao 266021;EFFECTS OF TEMPERATURE ON ACTIVITY OF HUMAN PLACENTAL ALKALINE PHOSPHATASE[J];Acta Acadimae Medicinae Qingdao;2002-04
2 Geng Fangsong, Wang Xiuli, Zhang Shehua, et al Department of Biochemistry ,Qingdao Medical College,Qingdao 266021;THE INHIBITION EFFECT OF L LEUCINE ON HUMAN PLACENTAL ALKALINE PHOSPHATASE[J];;1998-01
3 王景琳;The Determination of Cytochrome P450After Hepatic Microsomal Preparation or not.[J];Journal of Changzhi Medical College;1996-02
4 JI Chang-Hua,ZHANG Xiao-Guang,SHENG Li-Qun,SU Cheng-Zhi and CHENG Su-Min Department of Biochemistry;Purification and characterization of alkaline phosphatases from rabbit liver and small intestines[J];Journal of The Fourth Military Medical University;1993-01
5 Chen Wandong; Wang Chengguo; Ma Yuhang; Lu Mingfeng; Guo Jiantao (Institute of biological Chemistry Medicine and Health of Zhenping County,Henan Province, Zhenping 474250);Protection of Pb Cellular Toxicity and Pb Immune Toxicity by Metallothionein[J];TRACE ELEMENTS SCIENCE;1998-04
6 QIN Jun fa 1,CHEN Xiang you 2,LI Zeng xi 3 (1.Shanghai Institute of Nuclear Research,The Chinese Academy of Scienes,Shanghai 201800,China; 2.Jinling Trace Element and Health Institute,Nanjing 210001,China; 3.Chinese National Analysis Centre of G;Rare Earth's Effects of Toxicology[J];Trace Elements Science;2002-05
7 WANG Jing-yun1, PENG Xiao-jun2, YANG Dong3, BAO Yong-ming1, AN Li-jia1, ZHENG Xue-fang1 (1. Department of Bioengineering; 2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China; 3. Northeast Forestry Unive;Application of a Novel Synthesized Azo-Dye Ligand for the Affinity Separation of Alkaline Phosphatase[J];Journal of Chemical Engineering of Chinese Universities;2001-06
8 YANG Qiao-yuan, DONG Sheng-zhang. Department of Environmental Health, Sun Yat-Sen University of Medical Science, Guangzhou 510080, China;Repairing Effects of Zinc-metallothionein on Cadmium-Induced Oxidative Damage of Liver and Kidney in Mice[J];Journal of Environment and Health;2002-04
9 SHEN Guomin, XIN Ze-hua, QIAO Zhi-gang, XU Cun-shuan (1. Key Laboratory of Henan Province for Bioengineering, Xinxiang 453002, China; 2. College of Life Sciences, Henan Normal University, Xinxiang 453002, China);Analysis of alkaline phosphatase (AKP) in twenty-four kinds of tissues and organs of Andrias davidianus[J];Henan Science;2003-03
10 Sun Hongzhe Chen Rong Che Chiming  (Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Hong Kong);Metals in Biology and Medicine[J];Progress In Chemistry;2002-04
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 WANG Guo-hong,GENG Jun-li,HUANG Xiang-cai (College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Effects of 9 Kinds of Metal Ions on Cellulase Activity of Tenebrio molitor L.and the Growth of Larvae[J];Journal of Fujian Normal University(Natural Science Edition);2009-03
2 TONG Yao1,2,YE Jin-shao1,2,YIN Hua1,2,PENG Hui1,2,CHEN Shuo-na1,2,QIN Hua-ming1,2,HE Bao-yan1,2(1.Department of Environmental Engineering,Jinan University,Guangzhou 510632,China;2.Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation,Department of Education of Guangdong Province,Guangzhou 510632,China);Effect of Heavy Metal Ions on Triphenyltin Enzymatic Degradation[J];Environmental Science;2011-03
3 ZHOU Jun1,3,ZHENG Wei1,3,LI Xiaoming1,2,3,*,YANG Qi1,3,CHEN Wei1,3,LUO Kun1,3 1.College of Environmental Science and Engineering,Hunan University,Changsha 410082 2.College of Environment,Guangxi University,Nanning 530004 3.Key Laboratory of Environmental Biology and Pollution Control(Hunan University),Ministry of Education,Changsha 410082;Solubilization of excess sludge by thermophilic enzymes:Effect of metal ions[J];Acta Scientiae Circumstantiae;2011-08
4 ZHANG Wei-qiong,NIE Ming,WANG Hui,WANG Zhuo-yan,XIAO Ming(College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China);Effect of metal ions on cell growth and antibiotic production of Pseudomonas fluorescens P13[J];Journal of Shanghai Normal University(Natural Sciences);2007-02
5 FU Peng,ZHANG Guo-wen,WANG Lin,HU Ming-ming(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China);Effects of Cu~(2+),Co~(2+) and Mg~(2+) on Interaction between Farrerol and DNA[J];Food Science;2011-19
6 YANG Feng-Xiao TANG Yun (College of Biological Sciences,China West Normal University,Nanchong 637002);Effect of Metal Ions on Cell Growth and Phenol Degradation of a Thermophilic Strain BF80[J];Microbiology;2008-06
7 WANG Xiao-ping *, YI Min, LIN Juan, LIU Hai-jiang, ZHOU Zeng-tong, ZHANG Xiu-li, YU You- cheng. *Dental School, Tongji University, Shanghai 200072, China;Influence of lanthanum chloride on the osteogenesis of dog tissue engineering bone[J];Chinese Journal of Stomatological Research(Electronic Version);2008-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 FENG Zhi-Xiang, ZHANG Shu-Gong , LIU Qi-MinYANG Kui-Yue, NI Jia-Zuan(Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chanchun, 130022)MIAO Jian, YOU Shu-Heng (Department of Biochemistry, Henan Medical University, Zhengzhou);Interactions of Rare Earth Ions with Bovine Blood Cu(Zn)-Superoxide Dismutase[J];Chemical Research In Chinese Universities;1995-07
2 HUANG Zhong-Xian;ZHENG Qi;GAO Hong-Ying;GU Wei-Qiang;LIU Fang(Department of Chemistry,Fudan University,Shanghai,200433);The Reactions Between Lanthanons and Metallothionein[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-02
3 YANG Bin-Sheng(Institute of Molecular Science, Shanxi University, Taiyuan, 030006)T. G. St.Pierre, J. Webb(Murdoch University, Perth WA6150, Australia);The Effect of Europium (Ⅲ) on Iron Uptake by Apoferritin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-05
4 Lu Guocheng et al.(Dept. of Environ.Health, Beijing Medical University, Beijing 100083);Hygienic Investigation of Different Rare Earth(RE) Mining Areas in China: RE Levels of Farmer's Natural Living Environment and Head Hair[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1995-04
5 ZHANG Peiqun; LU Xiangli; QIAN Qinfang; FENG Weiyue (Institute of High Energy Physics, the chinese Academy of Sciences, Beijing 100080)GUI Zhong ZHANG Geyou DAI Qin REN Xianyun LIANG XiufenZHANG Meiyun (Huhhot Health and Anti- epidemic Station, Huhhot,;Study of trace element contents in scalp hair of the juveniles from the arsenism area in Inner Mongolia[J];NUCLEAR TECHNIQUES;1998-06
6 Sun Hong-Ye; Liu Qun; Yao Guang-Qing; Wang Kui (Inorganic Chemistry Department,School of Pharmaceutical Sciences Beijing Medical University, Beijing 100083);Ce ̄(3+) Binding to G-Actin and Consequent Conformation Change and Polymerization[J];;1996-01
7 Chen Xu; Zhou Yuxiang; Xu Yumin (Dept. of Biol. Sci & Biotech., Tsinghua University Biomembrane and Biomembrane Engineering National Laboratory, Beijing, 100084, P. R. China);THE EFFECTS LIGHT RARE EARTH IONS (Ln ̄(3+))ON THE ACTIVITY OF PHOSPHODIESTERASE STIMULATED BY CALMODULIN[J];ACTA BIOPHYSICA SINICA;1996-03
8 Li Liping et al.(Dept .of Public Health, Shandong Medical College, Jinan 515031);Cytoxicity of carbon arc lamp dusts[J];JOURNAL OF HEALTH TOXICOLOGY;1994-02
9 Wang Lihua; Wang Shao; Tang Yuhuan; Nie Yuxiu; Sun Wenwei (Department of Biochemistry, Norman Bethume University of Medical Sciences, Changchun 130021,China);Effects of Low Doses of Samarium Chloride on Serum Hormone in Rats[J];JOURNAL OF THE CHINESE RARE EARTH SOCIETY;1996-02
10 Zuo Zhiying, Bo Yunhong, Yang Xiaoda, Wang Kui( Department of Inorganic Chemistry, School of Pharmaceutical Science, Beijing Medical University, Beijing 100083, China ), Gao Song( Colledge of Chemistry and Molecular Engineering, Peking University, Bei;Effect of Lanthanides on Oxygen Carrier Function of Hemoglobin and Their Interaction[J];JOURNAL OF THE CHINESE RARE EARTH SOCIETY;1996-04
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