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《Journal of Beijing Medical University》 2003-02
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Effects of lanthanum on alkaline phosphatase

HU Jian, JIA Yu, LIU Xiang Tao △, LI Rong Chang, WANG Kui (Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, Beijing 100083,China)  
Objective: To investigate the effects of lanthanum on the activities of two kinds of alkaline phosphatase (AP) rat liver AP and bovine intestine AP,and the preliminary mechanism involved. Methods: A traditional colorimetric method was used to measure the activity of AP. The influence of La 3+ on the intrinsic fluorescence of protein was studied by the method of fluorescence titration into the AP solution by LaCl 3. The quenching equation was fitted based on the fluorescence spectrum, and then the binding number and the binding constants were calculated. Results: Lanthanum increasesd the activity of rat liver AP, whereas it decreased that of bovine intestine AP. It was observed that the binding of La 3+ quenched the fluorescence of bovine intestine AP, which indicated that La 3+ could change the conformation of AP in solution. The number of binding sites and the binding constants that were calculated based on the fitted quenching equation were 78 and 2.1×10 4 respectively. Conclusion: La 3+ has different effects on the activities of rat liver AP and bovine intestine AP; the binding of La 3+ can induce the changes of conformation of bovine intestine AP, and inhibit its activity.
【Fund】: 国家自然科学基金 (2 9890 2 80 )~~
【CateGory Index】: R341
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