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《Journal of Peking University(Health Sciences)》 2003-05
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Correlation of NDRG1 gene with liver tissue differentiation and hepatocarcinogenesis

HE Jing, ZHOU Rouli △ (Department of Cell Biology and Genetics, Peking University School of Basic Medical Sciences, Beijing 100083, China)  
Objective: To detect the expression profile of NDRG1 gene in different tissues and cell lines and explore the relationship of NDRG 1 with liver tissue differentiation and hepatocarcinogenesis. Methods: The expression profiles of NDRG 1 in hepatocellular carcinoma (HCC) tissues, paired noncancerous liver (PNL) tissues, adult normal liver (NL) tissues, baby mouse liver tissues and fetal liver tissues in different developmental stages and cultured cell lines were performed using RT PCR and Northern Blot analysis. Results: Expression of NDRG 1 was significantly up regulated in HCC tissues compared to that of NL tissues and PNL tissues, however, the expressions of NDRG1 in NL and PNL tissues showed no evident difference. In ten cell lines, the highest expression was in the 293T human kidney cell line, the next one in liver cell L02, and the lowest one in the undifferentiated HLE cell line. Expression of NDRG1 in liver tissues enhanced with the development of the baby mouse and human fetus. Conclusion: NDRG 1 is probably related to the liver cell differentiation and is highly expressed in the specific stage of the differentiation. However, it could not be recognized as a marker of differentiation. The high expression of NDRG 1 in HCC indicates that HCC is not just distributed to a simple de differentiation. Much more study is necessary in understanding the comprehensive relationship of the disorder proliferation and differentiation of HCC and the related genes.
【Fund】: 教育部教育振兴行动计划特殊专项 (“九八五”工程 );; 国家自然科学基金 ( 3 980 0 0 76)资助~~
【CateGory Index】: R735.7
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