Changes of matrix metalloproteinase 2,9 and tissue inhibitor of metalloproteinase1,2,3 expression level in aged rat lung
ZHAO Zhi-jie, LIU Xin-min, ZHOU Guo-peng, BU Ding-fang,LI Xue-ying, ZONG Li-li,LI Jiang(Department of Geriatris, Peking University First Hospital, Beijing 100034, China)
Objectives:To investigate the underlying mechanism of age related increase of pulmonary fibrosis incidence Methods: The levels of pulmonary collagen and TGF-β1 protein, MMP2,9 mRNA and TIMP 1,2,3 mRNA expression were measured by ELISA and realtime PCR. Results: As compared with adult rats [(756±160) pg/g vs (1 000±246) pg/g, P0.05] the aged rats had more extracellular matrix components, increased TGF-beta1 protein level, lower (3.15±1.76 vs 0.17±0.13, P0.01) MMP2 mRNA expression and TIMP 1,2,3 mRNA expressions (TIMP1 2.00±1.74 vs 0.11±0.06,TIMP2 7.60±2.51 vs 2.69±1.76, TIMP3 1.32±0.46 vs 0.29±0.16, P0.01). No difference was observed in MMP9 (1.66±0.67 vs 1.74±0.87, P0.05) mRNA expression. Conclusion: elevation in TGF-beta1 protein level, changes in the expression of MMP2/9 and decreased expression of TIMPS mRNA could be the underlying mechanism of age related increase of pulmonary incidence.
【Key Words】：
Aging Pulmonary fibrosis Transforming grouth factor beta Gelatinases Tissue inhibitor of metallo-proteinases
【Fund】： 国家自然科学基金项目资助(30300394)~~
【CateGory Index】： R563
【Fund】： 国家自然科学基金项目资助(30300394)~~
【CateGory Index】： R563
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