SEMI-QUANTITATIVE RT-PCR METHOD AND ITS APPLICATION IN THE STUDY ON GENE EXPRESSION

CHEN jiankang;ZOU Wanzhong;YOU Jiangfeng(Department of Pathology,Beijing Medical University 100083)  

Objective:Quantitative analysis of expression of endothelin B receptor(ETB-R)gene in rat heart,liver,lung,kidney and cuItured glomerular mesangial cells(MC)were observed. Methods:Se-mi-quantitative reverse transcription and polymerase chain reaction(SqRT-pCR)was used and com-pared with Northern blot hybridization. Results:EIB-R gene was expressed in all the above organs and MC,and highest expression level in the lung,moderate in the heart and kidney, but lowest in the liver. Conclusion:Different expression level of ETB-R gene in various organs imply that the effects of en-dothelin on different organs were not similar.The MC were target cells of endothelin in kidney.

【Key Words】： Headings Gene expression Polymerase chain reaction/methods Receptors endothelin
【Fund】： 国家自然科学基金
【CateGory Index】： Q3-3

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