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《JOURNAL OF BEIJING MEDICAL UNIVERSITY》 1998-06
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COMPARATIVE INVESTIGATION OF ANTHRAQUINONES IN HAIRY ROOTS, CALLI AND SUSPENSION CELLS OF RHEUM PALMATUM L.

CHANG Zhenzhan #, SHEN Xin, GUO De'an, LIU Lin, WANG Shasheng, ZHENG Junhua (#School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083)  
Objective: To compare the contents of free anthraquinones in Ri plasmid transformed roots, calli, suspension cells and wild roots of Rheum palmatum L.. Methods: Inducing hairy roots by infecting explants of Rheum palmatum L. using wild Agrobacterium rhizogenes 9402 strain, identifying transformed roots by Southern blot with rol C gene as probe. Inducing calli and suspension cells of Rheum palmatum L., respectively. Quantitatively determining the contents of six free anthraquinones——aloe emodin, rhein, chrysophanol, emodin, physcion, 8 O methylchrysophanol in hairy roots, calli, suspension cells wild roots of Rheum palmatum L. by high performance liquid chromatography(HPLC) technique. Results: The hairy roots were induced and the culture system was established. Result of Southern blot indicated that T DNA of Ri plasmid was transferred into the hairy root cells. The calli were induced from leaf fragments and the suspension cells were received by liquid culturing of the calli induced from leaf and stem fragments.The determination result showed that the content of each free anthraquinone in hairy roots was generally higher than that in calli, but lower than that in suspension cells or wild rhubarb roots. Conclusion: Production of anthraquinones by hairy root cultures is a valuable approach
【Fund】: 国家自然科学基金 国家教委博士点基金资助
【CateGory Index】: R931.6
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