Effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelial cells
LI Zhi Ping, LI Jin Lan, FU Jia Yu, CHEN Shan Shan (Institute of Hematology, People's Hospital, Beijing Medical University, Beijing 100034)
Objective: To observe the effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelia cells (HUVEC). Methods: Primary and passaged HUVEC were cultured in the medium with 20% mixed umbilical cord blood plasma and their purities were evaluated by Ⅷ:Ag and UEA Ⅰ.Proliferative activities of HUVEC were compared in the medium containing different amounts of mixed umbilical cord blood plasma by methyl thiazolyl tetrazolium(MTT) assay. Results: 20% mixed umbilical cord blood plasma can maintain cell viability in the medium without endothelial cell growth factor(ECGF). The purity of endothelial cells was 93.1%. Conclusion: 20 portion mixed umbilical cord blood plasma can be a substitute for ECGF and used successfully in the culture of HUVEC to obtain high purity and passagable endothelial cells.
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