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《Journal of Binzhou Medical University》 2009-02
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Effects of smokeless tobacco extract on the rat's osteoblasts in vitro

WANG Guilong HOU Yudong SHI Lingbo Department of Stomotology,Binzhou Medical University,Binzhou 256603  
Objective To investigate the effects of smokeless tobacco extract(ST) on the attachment and growth of the rat's osteoblasts in vitro.Methods The bone tissues from the calvaria in rats aged 1 week were cut into blocks of 1.5 mm × 1.5 mm.They were digested by 0.25% trypsinase for 20 minutes and by 0.1% collagenase II for 30 minutes and then the liquid supernatant was abandoned.The bone blocks were cultured to acquire isolated cells.The identification was conducted by cell morphology,histochemical staining of alkaline phosphatase(AL-Pase)and calcified nodules staining.The experiments were divided into control group and experiment groups.The experiment groups were divided into seven groups by the smokeless tobacco extract's concentration: 0.8,1.6,3.2,6.4,12.5,25 and 50 g/L,then the cell growth curves were drawn.Statistical analysis was performed using SPSS13.0 software package for two-way ANOVA.Results ST inhibited attachment and growth of osteoblasts in all groups with a concentration-dependent manner.The difference among all of groups was significant(P0.01);the difference between the same group was significant before 3 days(P0.01),while the difference among 3.2-50 g/L groups was no significant after 4 days(P0.05).Conclusion Smoking is harmful to the clinic healing of oral implant operation.
【CateGory Index】: R78
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