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《Journal of Chengdu University(Natural Science Edition)》 2009-04
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Extraction and Content Determination of Polysaccharide in Semen Brassicae Albae

LUO Wen,XU Haorui(School of Bioindustry,Chengdu University,Chengdu 610106,China)  
To investigate the effects of various extraction conditions,on the polysaccharide extraction yield of semen Brassicae Albae,such as extraction temperature,extraction time,ratio of liquid to material and thickness of powder,one factor and orthogonal experiment were made to determine the best extraction method and to determine the content of polysaccharide in it.Method of water solution and alcohol sedimentation was used to extract polysaccharides and phenol-sulfuric acid colorimetry was applied to determine the content of polysaccharides.The findings show that the best extraction is A3B2C3,that is at the temperature of 98 ℃,with a 1∶25 ratio of liquid to material and the time is two hours.Besides,findings of single-factor experiment indicate the best condition is twice of extraction,with a powder thickness of 40 and 5 times volumes of 95% ethanol.By this method,the extraction rate of polysaccharides is 3.821%,average recovery rate is 97.86.It is concluded that various factors have different effects on the extraction rate of polysaccharide of semen Brassicae Albae,the temperature is the most effective factor;the next is ratio of liquid to material and extraction time is least effective factor.
【CateGory Index】: R284.1
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