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《Journal of Changde Teachers University》 2001-03
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Possible Involvement of Calcium in Actinomycin D Induced Apoptosis in Maize

NING SHun-bin (Key Laboratory of MDE for Plant Developmental Biology, Wuhan University, Wuhan Hubei,430072)  
Calcium is involved in several steps of the apoptotic process in animals. Endonucleases are presumed to be the main targets of calcium; however, little is known about its role during plant programmed cell death. In this study, maize root meristematic tissues were submitted to treatment with cytotoxic reagent, the RNA-synthesis inhibitor Actinomycin D (ActD). The morphological and biochemical evidence for apoptosis in individual treated cells was identified. Further, the involvement of calcium has been detected using specific Ca 2+ -binging fluorescent dye, wazo-1. The results indicated that treatment with ActD resulted in an immediate increase in intracellular calcium. The calcium-mediated death process could be reversed before serious DNA breakage.
【CateGory Index】: S513
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【Co-references】
Chinese Journal Full-text Database 9 Hits
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