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《Journal of Hunan University of Arts and Science(Natural Science Edition)》 2010-03
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Content determination of Rutin in hibiscus mutabilis by HPLC

CHEN Zhen-gan,TANG-Xiao-mei,CHEN Yuan-dao,ZHOU Shi-biao(College of Chemistry & Chemical Engineering,Hunan University of Arts and Science,Changde 415000,China)  
To establish a method for content determinate of Rutin in Hibiscus mutabilis by HPLC.The chromatograp-hic column was C18 column(3.9×150 mm,4 um),methanol and 1% acetic acid solution(50/50,V/V) as mobile phase,at a flow rate of 1.0 mL/min,detection wavelength 254nm.Good linearity was obtained for Rutin and other components in the range of 4.16 ～ 41.6 mg/L peak area was favorable linear between the concentration of Rutin in range.The regression equation was A=15 086*C-26 310,correlation coefficient r = 0.999 3,The RSD of intra-day assay is within 5.32% ～ 6.02%.
【Key Words】： HPLC hibiscus mutabilis rutin
【Fund】： 湖南省教育科学“十一五”规划课题(XJK08BJG006)
【CateGory Index】： R284
【DOI】： CNKI:SUN:CDSZ.0.2010-03-017
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