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《Acta Agrestia Sinica》 1996-03
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Breakdown of Ribonucleic Acid with the Loss of Viability of Tall Fescue (Festuca arundinacea ) Seed

Pu Xinchun; Han Jianguo; Wang Pei ;Mao Peisheng' (Institute of Grassland Science, China Agricultural University, Bejing 100094)  
Aging, both before harvest and in storage after harvest, is the major determinant of the germination level and the physiological quality, sometimes was referred to as vigor, of a seed population, or seed lot. The germination level of a lot and the vigor of the germinable seeds interact with soil conditions eventually to determine whether or not a crop will be established suc cessfully. Total RNA, Poly A+ RNA were determined in tall fescue embryos during germination with accelerated aging for 0, 48 and 96hr. Both total RNA and Poly A+ RNA contents decreased with the loss of seed viability. The synthesis of new RNA, Poly A+ RNA was reduced signifi cantly during germination in aging seeds than non-aging seeds.. Gel electrophoresis of RNA showed that enhanced breakdown of both 25s and 18s RNA with loss of viability. It also reflected that, the decrease of germination rate of tall fescue aging seed was more due to the level of Poly A+ RNA than the level of total RNA.
【CateGory Index】: S54
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