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《ACTA AGRESTIA SINICA》 1998-02
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Study on the Genetic Diversity of the Alfalfa Local Varieties (Medicago sativa L. ) based on RAPD markers

Li Yongjun(The Center of Preservation and Utilization of Germplasm Resources of Animal Husbandry and Forage Grass,The Center of National Animal Husbandry & Veterinary Service, Beijing, 100094)Su Jiakai(Institute of Animal Science, CAAS, Beijing 10009  
Genetic diversity of the 18 alfalfa local varieties in China and the 9 alfalfa varieties representing the 9 alfalfa basic germplasm sources in North America were studied using RAPD markers.Resultsshowed that: Obvious polymorphisms within and among varieties can be revealed by amplifying individula DNA samples. Meanwhile, polymorphisms within varieties are concealed, and among varieties are obviously reduced,when using bulk samples.7 primers reveal total of 52 loci,and all the loci are scored as 1 forpresent and 0 for absent. Among the 52 loci,50 are polymorphic. Defference between varieties is reflected by the different frequencies on different loci. Total genetic diversity (HT) of the 18 alfalfa local varieties in China are close to, and polymorphic loci rate (P) and genetic diversity within varieties(HS)are higher than,that of the 9 alfalfa varieties in North America, indicating that the varieties in China are more heterozygous, and genetic variation within varieties are relatively larger. Analysis of gene differential coefficient (GST) indicating that genetic variation of Medicago sativa is mainly exits within varieties . This characteristic is related to the breeding system (out - breeing) of Medicago sativa.GST of the 18 alfalfa local varieties in China is smaller than that of the 9 alfalfa varieties in North America, indicating that more genecommunications occurred between the alfalfa local varieties in China, and variation within varieties takes up more of the total genetic variation. These results verify that the genetic structure of ch alfalfavarieties isalso related to their geographic distribution and sources. Polymorphisms revealed by RAPD markers arehigher than that of seed storage protein markers , indicating that polymorphism vary with different t levels of gene expression.
【CateGory Index】: S54
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