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《Acta Agrestia Sinica》 2002-03
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Plant Regeneration of Leymus chinensis Via in Vitro Culture

LIU Gong-she,WANG En-hua,LIU Jie *,QI Dong-mei,LI Fang-fang (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)  
The regenerated plants were obtained from Leymus chinensis for the first time through tissue culture.Immature-base explants were cultured on Murashige and Skoog's basal medium(MS) supplement- ed with 2 mg/L 2,4-D after surface sterilized with 0.1% HgCl 2 to induce callus at 25℃ constant temperature.After moved to MS medium containing 1mg/L 2,4-D for two times,the compact calluses were selected and transferred to MS basal medium containing 1.0mg/L Kt and 0.5mg/L NAA,which differentiated into shoot-buds. The shoot-buds appeared and regenerated new roots,finally developed plantlets on 2,4-D-free basal medium. Regenerated plants growth well when transferred into greenhouse.A comparative study of different explants,in this study,revealed that only could the callus from immature stachys differentiate into regenerated plants. The differentiate potential of regenerated plants has close related to the genotype and external hormone.
【Fund】: Key Project of the Chinese Academy of Science ( KSCX1-0 8-0 3) and State Key Project of theMinistry of Science and Technology( 2 0 0 1BA70 7B0 2 )
【CateGory Index】: Q943.1
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