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《Acta Agrestia Sinica》 2002-04
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A Preliminary Study on Self-incompatibility of Leymus chinensis

ZHANG Wei dong 1,LIU Gong she +1 ,LIU Jie 1,LIU Fan 2,QI Dong mei 1, LI Fang fang 1,DONG Gui jun 1,ZHAO Jing jing 1 (1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;2.Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China)  
The selfing and outcrossing seeding capability of wildrye ( Leymus chinensis )were investigated under the natural environments. The viability of pollens was estimated by the way of FDA fluorescent staining and the developmental characters of pistals and stamina at time and space were observed. The results showed that the selfing setting percentage was far less than the outcrossing setting percentage in the fields. The mean value of viable pollens was above exist temporal and spatial isolation between stigmas and anthers. In addition, thioredoxsin h gene was considered to be related to self incompatibility in poaceae plants. Using the primers designed from it, the anticipated fragment was detected in the genome of Leymus chinensis by PCR amplification. So, the authors considered that no or much lower selfing seeding percentage may lie in the self incompatible mechanism under the genetic control in Leymus chinensis , and the thioredoxsin h gene may be related to its self incompatibility. However, this is only an initial conclusion and much evidence is needed to prove this in cytology and molecular biology.
【Fund】: 中国科学院知识创新重大项目 (KSCX1-0 8-0 3 );; 国家十五攻关项目 (2 0 0 1BA70 7B0 2 )支持
【CateGory Index】: S543
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