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《Acta Agrestia Sinica》 2003-01
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A Study on Using RAPD in Alfalfa Salt Tolerant Breeding

Yang Qing-chuan 1, HAN Jian-guo 2 (1.Institute of Animal Science, CAAS, Beijing 100094, China; 2.Institute of Grassland Science, China Agicultural University, Beijing 100094, China)  
The salt tolerant alfalfa Zhongmu No.1 and the salt sensitive alfalfa Atlantic ocean are used as materials for extracting DNA, and polymorphic DNA molecular markers of 280 primers are selected through RAPD technology. Thirty-seven primers of polymorphic DNA have been found: OPW5、OPW8、OPW9、OPV4、OPV5、OPV9、OPM7、OPM8、OPM14、OPM18、OPT2、OPT3、OPT9、OPT11、OPT19、OPQ3、OPQ5、OPQ10、OPQ11、OPQ19、OPZ3、OPZ6、OPZ9、OPP5、OPP8、OPP20、OPK3、OPK8、OPK14、OPL13、OPO1、OPO2、OPO6、OPO9、OPO13、OPO16、OPO20. The study establishes for the research into the salt tolerant molecular genetic marker of alfalfa.
【Fund】: 国家高技术研究发展计划 (863计划 )项目 (2 0 0 2 AA2 4110 1)
【CateGory Index】: S541.9
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