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Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)

CHENG Lin-mei~1, CAO Qiu-fen~(1), GAO Hong-wen~(2*), HUANG Jing~1, MENG Yu-pin~1 (1.Shanxi Agri-Biotechnolgy Research Center, Taiyuan, Shanxi Province 030031, China; 2.Research and Development Center for Grassland Science, China Academy of Agriculture Sciences, Beijing, 10081, China)  
A research was conducted using leaves of Puna chicory as explants to establish a highly efficient Puna chicory regeneration system and its stable agrobacterium mediated transformation system. The result shows that the chicory regenerating frequency was increased from 48% to 100%, while the number of regenerating single leaf buds appearing as many as 12. After three days of culturing the chicory leaf seedlings with agrobacterium strain FIG 121 Hm, and two weeks in regenerate medium containing 20 mg/L kanmycin and 600 mg/L cephalosorine, the chicory leaf seedlings regenerated into transgenic buds, with the regenerating rate reaching 8.3%. The buds could develop in a medium of 30 mg/L kanamycin and reproduce successively, which is tentatively determined to be transgenic buds.
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