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《Acta Agrestia Sinica》 2004-03
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Genomic DNA Extraction and Optimizing RAPD Procedure of Lespedeza floribunda

ZHANG Ji-yu~1,YUAN Qing-hua~2,ZHANG Wen-shu~2,LU Ting~3 (1. College of Pastoral Agriculture Science and Technology of Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020 China;2.Institute of Animal Science, CAAS,Beijing 100094 China; 3.Grassland College of Gansu Agriculture University, Lanzhou 730070, China)  
A RAPD analysis was studied with the genomic DNA extraction of Lespedeza floribunda from its leaves at the flowering period,its dried seed,and its germinating seed using the CTAB method and the SDS method.An optimal reaction system and conditions are composed by 75 ng template DNA, 2.1 mmol/L Mg~(2+), 200 μmol/L dNTP,0.2 pm·μL~(-1),10×buffer 2.5 uL, 1.5 U TaqDNA polymerase,25 uL total reaction buffer. Thermal reaction program suitable for the RAPD analysis was devised as followings: an initial denaturation 94℃ for 3 minutes;45 cycles of 94℃ for 30 seconds,36℃ for 30 seconds,72℃ for 1 minute;and a final extention at 72℃ for 10 minutes。
【Fund】: 国家"十五"科技部基础性工作专项资助项目(2001~2003年)
【CateGory Index】: Q943
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