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《Acta Agrestia Sinica》 2005-03
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Effects of Drought Stress on Induced Protein in the Different Drought Resistance Alfalfa Leaf

KANG Jun-mei~1, YANG Qing-chuan~1,FAN Fen-cheng~2 (1.Institute of Animal Science, CAAS, Beijing 100094,China; 2.Grassland Institute, China Agricultual University, Beijing 100094,China)  
Using SDS-PAGE electrophoresis analytic method, a study on the change of drought-induced protein in leaves of alfalfa cultivars under different extents of drought stress was conducted. The result showed that there was a direct correlation between the change of drought-induced protein and drought stress intensity. As drought intensity increased gradually, the drought-induced protein increased initially. and then decreased . The amount of drought-induced protein varied among the different alfalfa cultivars. Those with strong drought-resistance could induce gene to produce more protein to adapt to the drought, while those lacking in drought-resistance would be weakened or lose self-adjustment. Thus, less drought-induced protein was produced. All the strong drought-resistance cultivars, after 15 days under drought stress, were induced to produce a new soluble protein pattern, while only CW300 and Archer Ⅱamong those weak in drought-resistance gave soluble protein pattern. All this indicates that the different cultivars possess distinct genetic diversities. Strong drought-resistance is an important property for breeding new cultivars.
【Fund】: “十五”国家高技术发展计划(863计划)(2002AA241101)
【CateGory Index】: S541.9
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